LCMS-IT-TOF法快速分析薄荷黄酮部位的主要化学成分  

Rapid analysis of chemical compositions in the flavonoid fraction of Mentha haplocalyx Briq. with LCMS-IT-TOF

作　　者：陈向阳[1] 张乐[1] 吴莹[2] 王卫华[3] 刘斌[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京中医药大学基础医学院 [3]中国计量科学研究院

出　　处：《北京中医药大学学报》2015年第8期546-550,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81173520);北京中医药大学研究生自主课题项目(No.2015-jxs-493)

摘　　要：目的快速分析鉴别薄荷黄酮部位的化学成分。方法采用高效液相色谱-离子阱-飞行时间质谱法(LCMS-IT-TOF)对薄荷黄酮部位化学成分进行分析,Sunfire C18色谱柱(4.6 mm×150mm,5μm),甲醇(A)-水(B)为流动相进行梯度洗脱,电喷雾离子源为质谱离子源,同时采用正、负离子检测模式,获得不同化合物的多级质谱数据。结果根据高分辨质谱结果和MS/MS碎片信息,结合对照品质谱信息及相关文献,共鉴定推断出12个黄酮类化合物,其中4个黄酮苷:芹菜素-7-O-芸香糖、香叶木苷、木犀草素-7-O-芸香糖、蒙花苷;6个黄酮苷元:5,6-二羟基-7,8,3',4'-四甲氧基黄酮、栀子黄素B、5,6-二羟基-7,8,4'-三甲氧基黄酮、刺槐素、5-羟基-6,7,8,3',4'-五甲氧基黄酮、香叶木素;1个黄酮醇苷:芦丁;1个二氢黄酮苷:橙皮苷。结论 LCMS-IT-TOF能快速有效地分析薄荷黄酮部位的化学成分,为其进一步研究提供物质基础。Objective To rapidly analyze chemical compositions in the flavonoid fraction of Mentha hap-localyx Briq.Methods The liquid chromatography-mass spectrometry-ion trap-time-of-flight （LCMS-IT-TOF）method was applied to analyze the flavonoid fraction of Mentha haplocalyx Briq.The chromato-graphic separation was performed on Sunfire C18 column（4.6 mm ×150 mm,5 μm）with gradient elution of methanol （A）-water （B）.Multistage mass spectrometry data of different compounds was obtained by using electrospray ionization source and monitoring in positive and negative ion modes.Results Twelve flavonoids,including four flavonoid glycosides,six flavonoid aglycones,a flavonol glycoside and a flava-none glycoside,were identified based on the characteristic MS /MS fragment ions data with chemical standards and published literatures.These constituents are mainly hesperidin,rutin,apigenin-7-O-ruti-noside,diosmin,luteolin-7-O-rutinoside,linarin,5,6-dihydroxy-7,8,3′,4′-tetramethoxy-flavone,gar-denin B,5,6-dihydroxy-7,8,4′-trimethoxy flavone,acacetin,5-hydroxy-6,7,8,3′,4′-pentamethoxy fla-vone and diosmetin.Conclusion LCMS-IT-TOF is a rapid and effective technology to obtain the chemi-cal compositions in the flavonoid fraction of Mentha haplocalyx Briq.,which is of great significance for further study.

关 键 词：薄荷 黄酮部位 高效液相色谱-离子阱-飞行时间质谱法 

分 类 号：R284.1[医药卫生—中药学]