一种新型介孔二氧化硅-硫化氢控释纳米微球的构建和评价  

Construction and Assessment of A Novel Control-Releasing Hydrogen Sulfide System Based on Mesoporous Silica Nanoparticles

作　　者：过常发[1] 王春生[1] 王文硕[1] 孙笑天[1,2] 

机构地区：[1]复旦大学附属中山医院心外科,上海市200032 [2]复旦大学附属华山医院心胸外科,上海市200040

出　　处：《中国分子心脏病学杂志》2015年第4期1402-1406,共5页Molecular Cardiology of China

基　　金：国家自然科学基金资助(81270326)

摘　　要：目的目前的硫化氢(H2S)研究仍缺乏理想的供体。本研究旨在利用介孔二氧化硅纳米粒子(MSN)合成一种新型的控释H2S供体,并评价其在体外实验中释放H2S的特点。方法溶胶-凝胶法制备均一大小的MSN,将二烯丙基三硫化物(DATS)负载至MSN孔道中,合成控释H2S系统(DATS-MSN)。分析其表征、释放H2S的特点、细胞毒性和细胞摄取能力。结果 DATS-MSN可在体外缓慢、持续、可调节的释放H2S。其可被心肌细胞成功摄取,在常规应用的浓度范围内未见明显细胞毒性。结论 DATS-MSN可在体外环境中缓慢而可控的释放H2S,并具备良好的体外安全性,是H2S体外研究的良好工具。Objective Hydrogen sulfide （H2S） acts many biological functions in various systems, however, H2S research still calls for an ideal donor slowly and controllably releasing H2S. This study is aimed to utilize mesoporous silica nanoparticles （MSN） as the carrier of diallyl trisulfide （DATS）, and synthesize a novel control-releasing H2S system. Methods The synthesis of MSN was carried out by sol-gel method, and DATS was successfully loaded. The characteristics of DATS-MSN were evaluated. In vitro, H2S release from DATS-MSN was assessed. Cytotoxicity assays and cellular uptake ability of DATS-MSN were evaluated using rat cardiomyocytes, Results A novel glutathione （GSH） activated control-releasing H2S system （DATS-MSN） had been synthesized. DATS-MSN （100 μg/mL） released moderate amounts of H2S in PBS slowly and continuously, and this process could bc regulated by changing concentration of GSH. Moderate quantities of nanoparticles could be uptaken by cardiomyocytes in vitro, and DATS-MSN showed little cytotoxicity in vitro when using as H2S donor. Conclusions DATS-MSN can slowly and controllably release moderate amounts of H2S in vitro, which showed little cytotoxicity and good uptake ability by cardiomyocytes. It provides researchers an ideal tool in in vitro studies.

关 键 词：硫化氢 介孔二氧化硅纳米粒子 二烯丙基三硫化物 控释 

分 类 号：R943[医药卫生—药剂学]