不同形态氮素配施对娃娃菜根系及氮素代谢相关酶活性的影响  

Effects of Combined Application of Different Forms of Nitrogen on Root and Nitrogen Metabolizing Enzymes Activity of Mini Chinese Cabbage

作　　者：杨兵丽 郁继华[1] 张国斌[1] 周箬涵 胡琳莉[1] 薛娟[1] 王博[1] 

机构地区：[1]甘肃农业大学园艺学院,甘肃兰州730070

出　　处：《河南农业科学》2015年第9期36-39,共4页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金(31260473);国家大宗蔬菜产业技术体系项目(CARS-25-C-07);甘肃省自然科学基金(145RJZA201)

摘　　要：设置10∶0、7∶3、5∶5、3∶7、0∶10的硝铵比,100%尿素处理,当地施肥和不施肥处理,分析不同氮素形态配施对娃娃菜根系及氮素代谢相关酶活性的影响,以期为探明娃娃菜氮素营养的调控机制及提高氮素营养的利用效率提供理论依据。结果表明,全部施硝态氮时地上部干物质积累量最大,随着铵态氮比例增加地上部干物质积累量总体降低;硝铵比在(10∶0)^(5∶5)时娃娃菜地下部干质量较大,以硝铵等比配施时最大。硝铵比3∶7时的总根长、根系总表面积和根尖数高于其他处理,说明硝铵比3∶7时植株能更好地吸收土壤中的养分,也更有利于侧根的发生;硝铵比5∶5处理的根系总体积和平均直径大于其他处理,但根尖数最少,根系生长最健壮。硝铵比在(10∶0)^(5∶5)时,氮素代谢关键酶活性、游离氨基酸含量较高,更有利于氮素的代谢利用。The treatments,10∶0,7∶3,5∶5,3∶7,0∶10 of nitrate ammonium ratios,100% urea treatment, local fertilization and blank as control were designed to investigate the effects of combined application of different forms of nitrogen on root and activities of enzymes related to nitrogen metabolism in Mini Chinese cabbage（ Brassica pekinensis） .The results showed that plants treated with only nitrate had maximum accu-mulation of shoot dry matter,and the accumulation of shoot dry matter reduced with the increase of the proportion of ammonium nitrogen.The root dry matter of plants fertilized with nitrate nitrogen∶ammonium nitrogen of 5∶5 reached the highest,and that of （10∶0）—（5∶5） reached higher.Total root length, root surface area and root tips number of plants treated with nitrate nitrogen∶ammonium nitrogen of 3∶7 were higher than those of plants under other treatments, indicating that plants treated with nitrate nitrogen∶am-monium nitrogen of 3∶7 had more lateral root and easily absorbed more water and nutrients.The root volume and average diameter of plants treated with nitrate nitrogen∶ammonium nitrogen of 5∶5 were greater than those of plants under other treatments,indicating that the growth of root was rather robust,although＆amp;nbsp;the number of root tip was the minimum under this treatment.When the nitrate nitrogen∶ammonium nitro-gen ranged from （10∶0）—（5∶5）,free amino acids and activities of key enzymes involved in nitrogen me-tabolism were higher,so it was more beneficial for plant nitrogen metabolism.

关 键 词：氮素配施 娃娃菜 硝酸还原酶 谷氨酰胺合成酶 转氨酶 

分 类 号：S143.1[农业科学—肥料学] S634.1[农业科学—农业基础科学]