痛风消停汤对AGA大鼠踝关节炎性因子表达的影响  

Effect of Tongfeng Xiaoting Decoction on Expression of Inflammatory Factors of Ankle Joint of AGA Rats

作　　者：周天寒[1] 黄学宽[2] 张超男[2] 邓福忠[1] 王志红[1] 杨治国[1] 王玲[2] 杨玉[2] 

机构地区：[1]重庆医药高等专科学校,重庆401331 [2]重庆医科大学中医药学院,重庆401331

出　　处：《实用中医药杂志》2015年第10期888-890,共3页Journal of Practical Traditional Chinese Medicine

基　　金：重庆市卫生局科技项目(2010-2-25)

摘　　要：目的:研究痛风消停汤对急性痛风性关节炎(AGA)大鼠受试踝关节滑膜组织炎性因子的影响。方法:60只SD雄性大鼠随机分为正常组、模型组、秋水仙碱组,痛风消停汤低、中、高剂量组,每组10只。正常组常规喂养,其余各组采用尿酸钠(MSU)溶液注射法建立急性痛风性关节炎模型。造模前2日,正常组与模型组按20m L/kg予以生理盐水灌胃,秋水仙碱组0.3mg/kg灌胃,1日1次;痛风消停汤低、中、高剂量组分别按2g/kg、4g/kg、8g/kg体质量给予痛风消停汤灌胃,1日1次,直至造模后第7日,连续9日。实验结束后,检测血尿酸,采用ELISA法检测受试踝关节IL-1β、IL-6、TNF-α的含量,免疫组化法检测TGF-β及其受体(TGF-βR1)的表达。结果:与正常组比较,模型组大鼠血尿酸、受试踝关节滑膜组织中IL-1β、IL-6、TNF-α含量明显升高(P<0.05);与模型组比较,痛风消停汤各组大鼠血尿酸、受试踝关节滑膜组织中IL-1β、IL-6、TNF-α含量明显降低(P<0.05),而痛风消停汤各组TGF-β1及TGF-βR1的表达较其他各组明显增强(P<0.05)。结论:痛风消停汤治疗急性痛风性关节炎的机制可能与上调TGF-β1及TGF-βR1的表达、抑制IL-1β、IL-6、TNF-α的表达有关。Objective： To investigate the effect of Tongfeng Xiaoting Decoction on the inflammatory factors in ankle joint synovial tissue of acute gouty arthritis rats（AGA）.Method： 60 male SD rats were randomly divided into 6 groups： normal group, model group, colchicine group and low, medium and high Tongfeng Xiaoting Decoction groups （10 in each group）.AGA model was established by induced monosodium urate （MSU） method except the normal group. Two days before AGA model was established, rats in normal and model groups received intra-gastric normal saline（NS） by 20ml/kg.d for qd, colchicine group received colchicine by 0.3mg/kg.d for qd, while those in low, medium and high Tongfeng Xiaoting Decoction groups received Tongfeng Xiaoting Decoction at the liquid of 2g/kg,4g/kg and 8g/kg respectively, once daily, until 7 days after model was formed, continuously for 9 days. After the experiment, serum uric acid was detected, the expression of IL-1β , IL-6, TNF- α , TGF- β 1 were detected by ELISA and the expression of TGF- β 1 and TGF- β R1 were detected by immunohistochemistry. Result： Serum uric acid and the content of IL-1β, IL-6, TNF- α, TGF- β 1 in ankle joint synovial membrane were increased significantly in the model group compared to that of the normal group （ P〈0.05 ） .Serum uric acid and the content of IL-1 β , IL-6, TNF- α, TGF- β 1 in ankle joint synovial membrane were decreased significantly in Tongfeng Xiaoting Decoction groups compared to that of the model group （ P〈0.05 ） . The expression of TGF- β land TGF- β R1 in ankle joint synovial membrane were increased significantly in Tongfeng Xiaoting Decoction groups compared to that of the others group （ P〈0.05 ） .Conclusion： The mechanism of treating gouty arthritis by Tongfeng Xiaoting Decoction may be through up-regulating the expression of TGF- β 1 ,TGF-r β 1 and inhibiting the expression of IL-1 β , IL-6, TNF- α in synovial tissue.

关 键 词：痛风消停汤 大鼠 急性痛风性关节炎 炎性因子 

分 类 号：R255.689.7[医药卫生—中医内科学]