缺氧及复氧对hepG2细胞STC-1和STC-2的mRNA表达影响  

Influence of anoxia-reoxygen for expression of STC-1 and STC-2 mRNA in hepG2 cell

作　　者：吴子安[1] 谭志容[2] 徐宁[1] 李涛[1] 李曼[1] 尹芳芳[2] 

机构地区：[1]广东省中医院芳村医院,广东广州510370 [2]广州中医药大学第二临床医学院,广东广州510405

出　　处：《实验技术与管理》2015年第8期58-61,共4页Experimental Technology and Management

基　　金：广东省医学科研基金立项课题(B2013169)

摘　　要：目的:研究缺氧及复氧对hepG2细胞STC-1和STC-2的mRNA表达影响。方法:用化学缺氧剂氯化钴模拟缺氧环境,用RT-PCR法分别检测在hepG2细胞缺氧和复氧条件下STC-1、STC-2的mRNA表达情况。结果:在hepG2细胞中,STC-1mRNA无论在缺氧还是复氧情况下,几乎不表达;STC-2mRNA在缺氧条件下表达升高,并随着缺氧时间延长而升高,复氧后表达又降低,并随复氧时间延长而恢复至正常。结论:STC-2与肝癌的发生发展过程密切相关。Objective To reserch the influence of anoxia‐reoxygen for the expression of STC‐1 and STC‐2 mRNA in hepG2 cell .Methods Using CoCl2 to simulate an anoxia environment ,then detecting the expression of STC‐1 and STC‐2 mRNA of hepG2 cell in different timing during the process of anoxia‐reoxygen .Results There is no expression of STC‐1 mRNA in hepG2 cell during the whole process of anoxia‐reoxygen .But there is an expression of STC‐2 mRNA in hepG2 cell when it is anoxia .The degree of the expression will be increased for the length of the time of anoxia and returned to normal after reoxygen .Conclusion There is a close correlation between STC‐2 and liver cancer in occuring and developing process .

关 键 词：HEPG2细胞 人斯钙素-1 人斯钙素-2 缺氧 复氧 STC-1 STC-2 

分 类 号：Q57[生物学—生物化学]