花荵叶片愈伤组织的诱导  

Callus Induction from Leaves of Polemonium coeruleum

作　　者：刘紫微[1] 赵鑫[1] 焦孟月 张彦妮[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2015年第9期45-47,共3页Journal of Northeast Forestry University

基　　金：黑龙江省留学归国基金项目(LC201410)

摘　　要：以花荵(Polemonium coeruleum)叶片为外植体,研究了不同消毒方法对叶片白化率的影响、不同激素种类及激素组合对叶片愈伤组织诱导的影响。结果表明:较适合花荵叶片消毒的方法是质量分数为75%的酒精消毒30 s后、质量分数为1%的Na Cl O消毒6 min,叶片白化率低,存活率较高。叶片愈伤组织诱导最佳培养基:MS+1.0 mg·L-16-BA(6-苄氨基腺嘌呤),诱导率为88.9%。We used the leaves of Polemonium coeruleum to study the effects of different disinfection method, different hormone type and hormone combination on callus induction from leaves. The best disinfection method was with 75% ethanol for 30 s and 0. 1% NaC10 for 6 rain, the leaf bleaching rate was low, and the survival rate was high. Optimal callus induction medi- um was MS medium with 1.0 mg · L^-1 6-BA with the induction rate of 88.9%.

关 键 词：花荵 叶片愈伤组织 诱导培养基 叶片白化率 

分 类 号：Q943.1[生物学—植物学]