山苦茶对动脉粥样硬化大鼠内皮祖细胞的保护作用  

Protective effect of Mallotus furetianus on function of endothelial progenitor cells in atherosclerotic rats

作　　者：刘月丽[1] 周丹[1] 林连波[1] 杨承[2] 

机构地区：[1]海南医学院药学院,海南海口571199 [2]海南医学院附属医院,海南海口571101

出　　处：《海南医学院学报》2015年第11期1453-1454,1458,共3页Journal of Hainan Medical University

基　　金：海南省自然科学基金(NO:813186)~~

摘　　要：目的:研究山苦茶对动脉粥样硬化(AS)大鼠内皮祖细胞(EPCs)的保护作用。方法:腹腔注射维生素D3和高脂饮食建立AS大鼠模型,预防性给予山苦茶高、低剂量(0.081g/kg、0.026g/kg)处理9周。处死大鼠,取骨干内骨髓,制成细胞悬液,梯度离心分离单个核细胞,培养诱导其分化成EPCs,并测定EPCs增殖、粘附、迁移和血管形成能力。结果:模型组大鼠EPCs的增殖、粘附、迁移和体外血管形成能力均显著低于正常组大鼠(均P<0.01),山苦茶提取物高剂量和维生素E组大鼠的EPCs的增殖、粘附、迁移和体外血管形成能力均显著高于模型组大鼠(P<0.05、P<0.01),山苦茶提取物低剂量组大鼠EPCs的迁移和体外血管形成能力高于模型组大鼠(P<0.01),结论:山苦茶可以保护AS大鼠EPCs功能。Objective： To investigate protective effect of Mallotus furetianus on function of endothelial progenitor cells （EPCs） in atheroselerotic rats. Methods： Atherosclerosis was established by intraperitoneal injection of vitamin D3 combined with high fat diet for 9 wk. Extracts of Mallotus furetianus were given to prevent damage of EPCs in dose of 0. 081 g/kg and 0. 026 g/kg. Rats were sacrificed, and then mononuelear cells were isolated from bone marrow to culture EPCs and test the functions of EPCs. Results.. Mallotus furetianus could improve the capacities of EPCs on proliferation, migration, adhesion, and tubule formation in doses of 0. 081 g/kg; improve adhesion, and tubule formation in dose of 0. 026 g/kg in atherosclerotic rats. Conclusion： Mallotus furetianus can protect functions of EPCs in atherosclerotic rats.

关 键 词：山苦茶 动脉粥样硬化 内皮祖细胞 大鼠 

分 类 号：R259.435[医药卫生—中西医结合]