8-硝基白杨素诱导人肝癌HepG2细胞分化研究  

Study on BrMC induced differentiation of human hepatocarcinoma HepG2 cells

作　　者：何贵成 严思佳[1] 丁恩 艾小红[1] 

机构地区：[1]南华大学附属第一医院肿瘤内科,湖南衡阳421001

出　　处：《现代医药卫生》2015年第19期2892-2895,共4页Journal of Modern Medicine & Health

基　　金：湖南省中医药管理局项目(201140)

摘　　要：目的探讨传统中药蜂胶的有效成分白杨素的人工半合成类似物8-溴-7-甲氧基白杨素,即8-硝基白杨素(BrMC)是否具有诱导人肝癌细胞分化作用。方法体外培养人肝癌Hep G2细胞,分别加入1.0μg/m L BrMC和1.0μg/m L全反式维甲酸(ATRA),同时设溶媒对照组和空白对照组。瑞氏-姬姆萨染色法检测BrMC诱导Hep G2细胞形态与核质比的变化;酶促反应试剂盒检测BrMC和ATRA诱导Hep G2细胞中碱性磷酸酶(ALP)和γ-谷氨酰转肽酶(γ-GT)的活性;放射免疫法检测甲胎蛋白(AFP)的质量分数;Diamondstone分光光度法(速率法)检测酪氨酸转氨酶(TAT)的活性。结果1.0μg/m L BrMC处理的细胞核质比远低于溶媒对照组,差异有统计学意义(P<0.01)。1.0μg/m L BrMC、ATRA组处理24、48、96 h的Hep G2细胞液中AFP质量分数,γ-GT、ALP、TAT活性分别与溶媒对照组比较,差异均有统计学意义(P<0.01)。结论 BrMC能降低Hep G2细胞核质比、减少Hep G2细胞的AFP分泌、有效地活化Hep G2细胞TAT、ALP;且BrMC和ATRA均能抑制Hep G2细胞γ-GT活性。BrMC通过以上作用诱导人肝癌细胞分化。Objective To evaluate whether the artificial semisynthetic analogue 8-bromo-7-methoxychrysin（Br MC）, i.e., 8-nitrochrysin as an effective component of traditional Chinese medicine propolis,having the ability for inducing the differentiation of human hepatocarcinoma cells. Methods The human hepatocarcinoma HepG2 cells were cultured in vitro. The Wright s-Giemse mixed coloring method was adopted to detect the changes of the cellular morphology and the nuclear-cytoplasmic ratio of HepG2 cells induced by Br Mc;the changes of microtubules microfilament protein array of HepG2 cells induced by Br MC and all trans retinoid acid（ATRA） were observed by Coomassie brilliant blue staining;the contents of alkaline phosphatase（ALP） and gammaglutamyl transpeptidase（γ-GT） in HepG2 cells were detected by the enzyme catalyzed reaction;the radioimmunoassay（RIA） was used to detect the mass fraction of alpha fetal protein（AFP）;the content of tyrosine transaminase（TAT） was measured by the Diamondstone spectrophotometry. Results The cytoplasmic ratio by 1.0 ug/m L Br MC handled was far lower than that of solvent con trol group,the difference was statistically significant（P〈0.01）.1.0 μg/m L Br MC and ATRA handled 24,48,96 hours,the mass fraction of AFP,activity of γ-GT,ALP,TAT compared with the solvent control group respectively,the difference were statistically significant（P〈0.01）. Conclusion Br MC induces human hepatocarcinoma cellular differentiation by lowing the nucleus-cytoplasmic ratio,decreasing the secretion of AFP,effectively activating TAT and ALP in HepG2 cells;moreover Br Mc and ATRA inhibiting the activity of γ-GT.

关 键 词：植物提取物 肝肿瘤/病理学 肿瘤细胞 培养的 8-硝基白杨素 HEPG2细胞 分化 诱导 

分 类 号：R979.1[医药卫生—药品]