眼镜蛇长链神经毒素镇痛效应及可能机制  

Analgesic effect of cobratoxin and its possible mechanism

作　　者：彭建明[1] 苏兰娣[1] 罗雪[1] 叶记林[1] 于有江[1] 

机构地区：[1]扬州职业大学医学院,江苏扬州225009

出　　处：《现代医药卫生》2015年第19期2896-2898,共3页Journal of Modern Medicine & Health

基　　金：江苏省卫生厅卫生职业技术教育基金资助项目(NJ201112);扬州环境资源学院校级课题(N201129)

摘　　要：目的探究眼镜蛇长链神经毒素(CBT)的镇痛作用及可能机制。方法采用在体细胞外记录方法记录慢性炎症痛模型大鼠脊髓背角神经元的痛诱发放电,分别对六组实验大鼠注射生理盐水(5μL)、CBT(20、40、80 ng/kg)、阿托品(2 mg/kg)、阿托品(2 mg/kg)+CBT(40 ng/kg),观察不同含量CBT对脊髓背角神经元痛诱发放电的影响,同时观察胆碱能受体拮抗剂阿托品能否翻转其镇痛效应。结果不同含量CBT均可以显著抑制慢性炎症痛大鼠脊髓背角神经元的痛诱发放电,各含量CBT组放电频率与生理盐水组比较,差异均有统计学意义(P<0.05),且该效应可被阿托品所阻断,阿托品+CBT组在20、30、40、50、60 min放电频率与CBT(40 ng/kg)组比较,差异均有统计学意义(P<0.05)。结论CBT具有显著的镇痛效应,该效应有一定的剂量依赖性,胆碱能受体系统参与了其镇痛过程。Objective To investigate the analgesic effect of cobratoxin（CBT） and its possible mechanisms. Methods The pain-evoked discharge from the spinal dorsal horn neurons in rats with chronic pain was recorded by the somatic extracellular record method. The 6 experimental rat groups were injected by the normal saline（5 μL）,CBT（20,40,80 ng/kg）,atropine（2 mg/kg）and atropine（2 mg/kg）＋CBT（40 ng/kg） respectively. The influence of different concentrations of CBT on the pain-evoked discharge from the spinal dorsal horn neurons was observed. At the same time,whether cholinergic receptor antagonist atropine overturning its analgesic effect was observed too. Results The different concentrations of CBT could obviously inhibit the pain-evoked discharge from the spinal dorsal horn neuron in rats with chronic pain,the discharge frequency had statistically significant difference between the different concentration and the normal saline group（P〈0.05）,moreover the effect could be blocked by atropine,the difference of the discharge frequency at 20,30,40,50,60 min had statistical difference between the atropine＋CBT group and the CBT 40 ng/kg group（P〈0.05）. Conclusion CBT possesses a significant analgesic effect,this effect has certain dose dependence and the cholinergic receptor system participates in this analgesic process.

关 键 词：眼镜蛇毒液类 眼镜蛇属 神经毒素类 镇痛 

分 类 号：R996.3[医药卫生—毒理学]