VPA对3T3细胞中多能性转录因子和miR-367的影响研究  

Effect of VPA on the expression of transcription factor and miR-367 in 3T3 cells

作　　者：许阁阁 钟原[1] 袁宝[1] 陈健[1] 任文陟[1] 张嘉保[1] 刘殿峰[1] 俞先峰[1] 

机构地区：[1]吉林大学动物科学学院,吉林长春130062

出　　处：《中国兽医学报》2015年第9期1472-1475,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31201866);高等学校博士学科点专项科研基金资助项目(20110061120100)

摘　　要：小分子化合物-丙戊酸(valproic acid,VPA)作为组蛋白去乙酰化酶抑制剂(histone deacetylase inhibitors,HDACIs),能够有效促进体细胞重编程的效率。为了探讨VPA对microRNA和多能性转录因子的影响,首先用MTT法确定VPA的浓度梯度,采用流式细胞术检测VPA对3T3细胞周期的阻滞情况,同时观察细胞形态的变化,并用荧光定量PCR法检测Oct4和Sox2以及miR-367表达量的变化。结果显示,VPA剂量依赖性地抑制3T3细胞的增殖,且随着浓度的升高抑制作用增强,并将细胞周期阻滞在G2/M期。此外,VPA能使Oct4和miR-367的表达量增加。这表明VPA在体细胞重编程中可能介导多能性转录因子Oct4和miR-367的表达来促进重编程效率。Small molecule compound- valproic acid （VPA） is a histone deacetylase inhibitor that can effectively increase the efficiency of somatic cell reprogramming. To investigate the effect of VPA on the expression levels of microRNA and pluripotency transcription factors,we have deter- mined the VPA concentration gradient by MTT assay,and detected the arrest of 3T3 celt cycle by flow cytometry,and observed the morphology of cells, and also detected the expression levles of Oct4,Sox2 and miR-367 by fluorescence quantitative PCR method. The results showed that the VPA dose-dependently inhibited the proliferation of aT3 cells, and the inhibition rate were en- hanced with increasing concentration, and cell cycle were arrested in G2/M phase. In addition, VPA can increase the expression of Oct4 and miR-367. These results suggest that VPA may mediate the pluripotency transcription factors Oct4 and miR-367 to enhance the reprograming efficiency.

关 键 词：丙戊酸(VPA) 重编程 转录因子 miR367 

分 类 号：S852.2[农业科学—基础兽医学]