蛋鸡不同产蛋期生殖器官中OC17,OC116和OCX32 mRNA相对定量研究  

Relative quantification of OC17,OC116 and OCX32 mRNA in reproductive organs of hens in different laying periods

作　　者：叶幼荣 孙杰[1] 张璐[1] 赵宗胜[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003

出　　处：《中国兽医学报》2015年第9期1518-1525,共8页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31060305);教育部高校博士点基金资助项目(201065181200003)

摘　　要：Ovocleidin-17(OC17),Ovocleidin-116(OC116)和Ovocalyxin-32(OCX32)是蛋壳中的特异性基质蛋白,对蛋壳的钙化有重要的调节作用。本试验采用RT-PCR、RT-QPCR分别检测了蛋鸡在产蛋初期、高峰期和后期的卵巢、输卵管的伞部、膨大部、峡部以及子宫部OC17、OC116和OCX32的表达量变化。结果显示:在不同产蛋期3个基因在输卵管峡部和子宫部的表达量均显著或极显著(P<0.05或P<0.01)高于其他组织;卵巢和输卵管中OC17、OC116和OCX32在产蛋高峰期,初期和后期的表达量变化存在显著或极显著差异(P<0.05或P<0.01);3个产蛋期OCX32在5个组织中的表达量均极显著高于OC17和OC116(P<0.01)。研究结果揭示了蛋壳特异性基质蛋白基因OC17、OC116和OCX32在不同产蛋期蛋鸡生殖器官中的表达规律,为蛋壳形成的分子机理研究提供了理论依据。Ovocleidin-17 （ OC17 ）, ovocleidin-116 （ OC116 ） and ovocalyxin-32 （ OCX32 ） are eggshell specific matrix proteins, these proteins have important roles in regulating eggshell mineralization. In this study, HyAAne brown laying hens were used to determine the expression changes of OC17, OC116 and OCX32 in ovary,ampullary,magnum tubae,isthmus and uterus of the fallopian tube in different laying periods （earlier period, peak period and late period of laying） by reverse tran- scriptase poIymerase chain reaction （RT-PCR） and quantitative real-time PCR （RT-QPCR）. The results showed that the expression levels of OC17,OC116 and OCX32 mRNA in isthmus and ute- rus were very significantly or significantly higher than those of other tissues in different laying pe- riods（P〈0.05 or P〈0.01）. The expression differences for OC17,0C116 and OCX32 in ovary and fallopian tube in three laying periods were very significant or significant （P〈0.05 or P〈0.01）. The expression levels of OCX32 mRNA in all five tissues in three laying periods were all very sig- nificantly higher than those of OC17 and OC116 （P〈0.01）. The results revealed the expression patterns of OC17,OC116 and OCX32 in reproductive organs of hens in different laying periods, Which provides theoretical basis for the research of molecular mechanism of egg shell formation.

关 键 词：蛋鸡 产蛋期 生殖器官 Ovocleidin-17 Ovocleidin-116 Ovocalyxin-32 

分 类 号：Q38[生物学—遗传学]