阿拉善驼妊娠前后卵巢差异蛋白质组学分析  

Comparative proteomics studies of pregnancy and non-pregnancy ovary tissue of Alashan Bactrian camel

作　　者：陶金忠[1] 赵国顺[2] 刘姗姗[3] 赵兴绪[2] 胡俊杰[2] 张勇[2] 

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]甘肃农业大学动物医学院,甘肃兰州730070 [3]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《中国兽医学报》2015年第9期1535-1538,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31001095)

摘　　要：为了比较阿拉善双峰驼妊娠前后卵巢中差异表达的蛋白质,采集非繁殖季节未妊娠和妊娠期阿拉善驼卵巢,运用双向电泳技术构建双峰驼卵巢蛋白质凝胶电泳图谱,凝胶经考马斯亮蓝染色后,用PDQuest 8.0软件检测3倍以上的表达变化的蛋白点。结果表明在两组样品中共检测到10个差异蛋白点,这些蛋白点经MALDI-TOF/TOF-MS鉴定为8种蛋白质,包括谷胱甘肽S-转移酶、过氧化物酶-6、丙酮酸激酶同工酶M1/M2异构体1、纤维蛋白原β链前体、核纤层蛋白A/C转录物变异体1、视黄醛脱氢酶1和一种未命名的蛋白质。其中核纤层蛋白A/C转录物变异体1仅在未妊娠卵巢中表达,视黄醛脱氢酶1在双峰驼妊娠卵巢组织中表达量下降,而其他蛋白质在妊娠卵巢中表达量增加。这些差异表达蛋白质的发现为了解双峰驼妊娠期卵巢的生理机制奠定了基础。The aim was to identify differentially expressed proteins in the ovary tissue collected from the Bactrian camel （Camelus bactrianus） which was divided into two groups according to their pregnancy condition and crude ovary tissue samples were analyzed by 2-dimensional gel elec- trophoresis （2-DE）. Differential protein spots were detected by PDQuest 8.0 software after stai- ning with commassie blue. Ten differentially expressed proteins were found from the pregnant and non-pregnant Bactrian camel ovary tissue. These spots were identified by MALDI-TOF/TOF-MS. Eight unique proteins was found including glutathione-S-transferase isozyme, catalase-6, pyruvate kinase isozyme M1/M2 isozymer 1, precursor of fibrinogen D-chain, nuclear lamina protein A/C transcript variant 1 and retinal dehydrogenase 1 et al. Among the eight proteins, the nuclear lamina protein A/C transcript variant 1 was only expressed in non-pregnant ovary, retinal dehydrogenase 1 was down-regulated in pregnant ovary,and all other proteins were up-regulated in pregnant ova- ry.

关 键 词：双峰驼 卵巢 非繁殖季节 妊娠期 双向电泳 

分 类 号：S852.2[农业科学—基础兽医学]