雌激素对奶牛输卵管上皮细胞中mPGES-1表达的影响  

Effect of estrogen on mPGES-1 expression in bovine oviduct epithelial cell

作　　者：毛伟[1,2] 董至恒[1,2] 黄娜[1,2] 张莹[1,2] 曹金山[1,2] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]农业部动物疾病临床诊疗技术重点实验室,内蒙古呼和浩特010018

出　　处：《中国兽医学报》2015年第9期1553-1556,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31160490);新世纪优秀人才计划资助项目(NCET-11-109);内蒙古自然科学基金资助项目(2010BS0406)

摘　　要：观察雌激素(β-雌二醇)对奶牛输卵管上皮细胞膜结合型前列腺素E2合酶-1(mPGES-1)表达的影响,并探讨β-雌二醇对奶牛输卵管上皮细胞合成分泌PGs调控的作用机制。采用胰酶消化法及机械法分离培养奶牛输卵管上皮细胞,应用荧光定量RT-PCR技术检测β-雌二醇对奶牛输卵管上皮细胞mPGES-1 mRNA表达的影响,应用Incell Western技术检测β-雌二醇对奶牛输卵管上皮细胞mPGES-1蛋白表达的影响。结果显示,与空白对照相比,β-雌二醇能够显著和极显著地促进奶牛输卵管上皮细胞中mPGES-1基因和蛋白的表达。本试验结果表明,β-雌二醇能够对奶牛输卵管上皮细胞中mPGES-1产生调节作用。The study aimed at observing effect of β-estradiol on mPGES-1 expression and investi- gate the mechanism on the regulation of β-estradiol on the synthesis and secretion of PGs in bovine oviduct epithelial cell. Bovine oviduct epithelial cells（BOECs） were isolated and cultured by me- chanical and pancreatic enzyme digestion method;mRNA expression of mPGES-1 was detected by real-time RT-PCR in bovine oviduct epithelial cells;protein production of mPGES-1 was detected by Incell Western. The results show that compared with the blank control,β-estradiol markedly promoted the mRNA and protein expression levels of mPGES-1 in bovine oviduct epithelial cells. It is concluded that β-estradiol can regulate the expression levels of mPGES-1 in bovine oviduct epi- thelial cells.

关 键 词：雌激素 奶牛输卵管上皮细胞 膜结合型前列腺素E2合酶-1 

分 类 号：S823[农业科学—畜牧学]