MyoG与Myf5基因与京海黄鸡生长性状的相关分析  

Association of growth traits with the MyoGand Myf5 genes in the Jinghai yellow chicken

作　　者：唐莹[1,2] 张跟喜[1,2] 樊庆灿[1,2] 王金玉[1,2] 张涛[1,2] 魏岳[1,2] 薛倩[1,2] 王文浩[1,2] 王永娟 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009 [3]江苏京海集团,江苏南通226103

出　　处：《中国兽医学报》2015年第9期1562-1567,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31201793);国家肉鸡产业技术体系资助项目(nycytx-42-G1-05);江苏高校优势学科建设工程

摘　　要：以京海黄鸡为材料,采用PCR-SSCP方法检测MyoG和Myf5基因的单核苷酸多态性(SNPs),并分析基因型间的互作以及基因型对生长性状的遗传效应。结果表明,MyoG基因外显子3上存在一个T36C突变,形成了AA、AB和BB 3种基因型;Myf5基因外显子1上存在一个A1313G突变,形成了CC、CD和DD 3种基因型。对生长性状的最小二乘分析结果显示,BB型个体的2~16周龄均显著或极显著的高于AA型个体(P<0.05或P<0.01)。CD型个体仅在初生体质量上显著优于CC型个体。基因互作分析结果显示,互作效应对6、8周龄体质量的影响达到了极显著水平(P<0.01)。本试验为京海黄鸡生长性状的标记辅助选择提供了参考依据。The aim of this study was to analyze the association of SNPs in MyoG and My f5 genes with growth traits in the Jinghai yellow Chicken. PCR-SSCP approach was used to detect the single nucleotide polymorphisms（SNPs） and gene interaction and genetic effects between different geno- types and growth traits were also analysed. For the MyoG gene, three genotypes （AA, AB and BB） were detected. Gene sequencing revealed one mutation （T36C） in the genotype BB in comparison to the genOtype AA^while for the My f5 gene,three genotypes （CC,CD and DD） were detected. Gene sequencing revealed one mutation （A1313G） in the genotype DD in comparison to the geno- type CC. The least square analysis showed that individuals with BB genotype had higher body- weight at 2 to 16 weeks compared to individuals with AA genotypes. Individuals with CD genotype had higher birth weight than individuals with CC genotype （P〈0.05）. Gene interaction made a difference in the 6-week weight and 8-week weight. This study may provide reference materials for marker-assisted breeding of growth traits in Jinghai yellow chicken.

关 键 词：京海黄鸡 MYOG基因 Myf5基因 基因互作 生长性状 

分 类 号：S831.2[农业科学—畜牧学]