HPLC法同时测定痛经贴剂中柚皮苷、橙皮苷和新橙皮苷的含量  

Simultaneous determination of naringin,hesperidin and neohesperidin in dysmenorrhea patches by HPLC

作　　者：张丽君[1,2] 田军[2] 刘永[2] 

机构地区：[1]安徽中医药大学,安徽合肥230012 [2]安徽省食品药品检验研究院安徽省中药研究与开发重点实验室,安徽合肥230051

出　　处：《安徽医药》2015年第10期1877-1880,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立HPLC法同时测定痛经贴剂中柚皮苷、橙皮苷和新橙皮苷的含量。方法采用高效液相色谱法,以CosmosilC18为色谱柱(5μm,4.6 mm×250 mm);乙腈—0.1%磷酸(21∶79)为流动相;流速为1.0 m L·min^(-1);检测波长为283 nm;柱温为25℃;进样量为10μL。结果柚皮苷浓度在10.63~340 mg·L^(-1),橙皮苷浓度在7.81~250 mg·L^(-1),新橙皮苷浓度在8.13~260 mg·L^(-1)范围内线性关系良好,平均加样回收率分别为98.01%、97.90%和98.60%。结论该方法简单、准确、可靠,可用于痛经贴剂的质量控制。Objective To establish a method for simultaneous determination of naringin,hesperidin and neohesperidin in dysmenorrhea patches by HPLC.Methods The HPLC method was validated using a Cosmosil C18 analytical column(5 μm,4.6 mm ×250 mm)and the mobile phase was acetonitrile -0.1% phosphoric acid (21∶79)at a flow rate of 1.0 mL·min -1 with UV detection at 283 nm.The column temperature was kept at 25℃ and the injection volume was 10 μL.Results The linear ranges of naringin,hesperidin and neo-hesperidin were 10.63 ~340 mg·L -1 ,7.81 ~250 mg·L -1 and 8.13 ~260 mg·L -1 ,respectively.The average sample recovery rates were 98.01 %,97.90 % and 98.60 %,respectively.Conclusion The method is simple,accurate and reliable,which can be used for quality control of dysmenorrhea patches.

关 键 词：高效液相色谱法 痛经贴剂 柚皮苷 橙皮苷 新橙皮苷 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]