苓桂术甘合三拗汤止咳、化痰、平喘作用的实验研究  

Experimental Study on Facilitating expectoration,Suppressing Cough and Relieving Dyspnea of San Ao Tang with Ling Guizhugan

作　　者：温瑞书[1] 宋翠淼[1] 邓国兴[1] 许华洲 吴中秋[1] 王文智[1] 陈小玉[2] 侯晓明[2] 

机构地区：[1]河北中医学院,石家庄050091 [2]天津港口医院

出　　处：《河北中医药学报》2015年第3期4-7,共4页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：河北省科技厅资助项目:No 06276102D-35

摘　　要：目的:观察苓桂术甘合三拗汤的止咳、化痰、平喘疗效。方法:⑴止咳作用:将小鼠分为苓桂术甘合三拗汤合方高、低剂量组、苓桂术甘汤高、低剂量组、三拗汤高、低剂量组、桂龙咳喘宁对照组、咳必清对照组、模型对照组。各组灌胃给药,用小鼠浓氨水引咳法观察苓桂术甘合三拗汤合方的止咳作用。⑵化痰作用:将小鼠随机分为模型对照组、苓桂术甘合三拗汤合方(简称合方组)高、低剂量组、苓桂术甘汤高、低剂量组、三拗汤高、低剂量组、桂龙咳喘宁对照组及氯化铵对照组,用小鼠气管段酚红排泌量法观察苓桂术甘合三拗汤合方的祛痰作用。⑶平喘作用:将预选合格的敏感豚鼠随机分为苓桂术甘合三拗汤合方组(简称合方组)、苓桂术甘汤组、三拗汤组、氨茶碱组、模型对照组。制备苓桂术甘汤和三拗汤水煎液及二者等容积混合液。除氨茶碱组腹腔注射给药外(因不起雾),其余各组喷雾给药,每天15 min,连喷3天,用2%氯化乙酰胆碱和0.1%磷酸组织胺等容积混合液致豚鼠哮喘,以观察豚鼠的诱喘潜伏期。结果:⑴止咳作用:苓桂术甘合三拗汤合方高剂量组的小鼠咳嗽潜伏期明显延长,与模型对照组比较,差异有显著性,P<0.05;与三拗汤高、低剂量组比较,潜伏期延长,但差异无显著性;与苓桂术甘汤高、低剂量组比较,潜伏期明显延长,差异有显著性,P<0.05。⑵化痰作用:对小白鼠气管段酚红排泌量测定显示,苓桂术甘合三拗汤合方高低剂量组和三拗汤高低剂量组、苓桂术甘汤高低剂量组均可增加小鼠气管段酚红排泌量,与模型组比较,差异有显著性,P<0.05。而以苓桂术甘合三拗汤合方高剂量组为更佳。⑶平喘作用:苓桂术甘合三拗汤合方组的哮喘潜伏期与模型组和苓桂术甘汤组比较,差异有显著性(P<0.05);与三拗汤组比较,差异有显著性(P<0.05)。结论:苓桂术甘汤合三拗汤�Objective：to observe the curative effect of San Ao Tang with Lingguizhugan on facilitating expectoration, suppressing cough and relieving dyspnea. Methods：1） suppressing cough：the mice were divided into high and low dosage groups of combined for-mula, high and low dosage groups of Lingguizhugan Tang, high and low dosage groups of San Ao Tang, Guilong Kechuangning Cap-sule group, Kebiqing group and control group. Each group was given intragastric administration to observe the function of suppressing cough by mouse concentrated ammonia-induced cough. 2） facilitating expectoration：the mice were randomly divided into model group, high and low dosage groups of combined formula, high and low dosage groups of Lingguizhugan Tang , high and low dosage groups of San Ao Tang, Guilong Kechuangning Capsule group and NH4CL （ammonium Chloride） group. The function of facilitating expectora-tion was observed by mice trachea phenolsulfonphthalein excretion quantity method. 3 ） relieving dyspnea： the preselected qualified sensitive guinea pigs were randomly divided into combined formula group, Lingguizhugan Tang group, San Ao Tang group, aminolhyl-line group and control group. Prepare water solutions of Lingguizhugan Tang and San Ao Tang , and mixed liquor of both solutions with the same volume. Except aminolhylline group with intraperitoneal injection, the others were administered with atomization, 15 min a day for three days. Mix the same volume of 2% acecoline and 0. 1% histamine phosphate to induce asthma so to observe the latency period in guinea pigs. Results：1） suppressing cough：the latency period in the high dosage group of combined formula was prolonged, compared with control group, with significant difference, P 〈0. 05;compared with both groups of San Ao Tang , the period was pro-longed without significant difference; compared with both groups of Lingguizhugan Tang , the period was prolonged with significant difference, P 〈0. 05. 2） facilitating expectoration：mice trachea phenolsulfonphth

关 键 词：苓桂术甘汤 三拗汤 咳嗽 哮喘 止咳 化痰 平喘 桂龙咳喘宁胶囊 

分 类 号：R285.5[医药卫生—中药学]