四极子DNA修饰金纳米棒的制备及其用于荧光光谱法测定铅(Ⅱ)  

Preparation of Gold Nanorods Modified by G-Quadruplex DNA and Its Application to Fluorospectrophotometric Determination of Pb(Ⅱ)

作　　者：甘浩[1,2] 邓留[1,2] 

机构地区：[1]中南大学化学化工学院,长沙410083 [2]中南大学有色金属资源化学教育部重点实验室,长沙410083

出　　处：《理化检验（化学分册）》2015年第9期1269-1273,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金资助项目(21175156;20975114;20775093);中国博士后基金资助项目(2011M500126;2012T50656)

摘　　要：采用四极子（G4）DNA修饰的金纳米棒通过荧光对铅（Ⅱ）进行检测。由于铅（Ⅱ）对四极子DNA结构具有极高的稳定效率,能够驱使四极子DNA形成四极子结构与荧光探针锌原卟啉（ZnPPIX）螯合。金纳米棒不仅作为DNA的载体,而且金纳米棒的荧光增强效应还可以提高ZnPPIX-G4的荧光强度,从而构建了一个灵敏的铅（Ⅱ）荧光传感器。以透射电子显微镜、荧光光谱法及紫外吸收光谱法等表征该四极子DNA-金纳米棒复合材料。该荧光传感器的线性范围为100nmol·L^-1以内和150~1 000nmol·L^-1之间,检出限（3S/N）为0.1nmol·L^-1。采用该检测器对自来水样品进行分析,测定结果与电感耦合等离子体原子发射光谱法的测定结果相符。Gold nanorods modified by G-quadruplex was used for the fluorescence determination of Pb（Ⅱ）.Pb（Ⅱ）can drive the formation of G-quadruplex DNA which can chelate with the fluorescent probes zinc protoporphyrin because Pb（Ⅱ）can stabilize the G-quadruplex DNA structure with high efficiency.The gold nanorods not only are a DNA carrier,but also enhance the fluorescence intensity of ZnPPIX-G4.Based on the fact,a new Pb（Ⅱ）sensitive fluorescent sensor was constructed. The G-quadruplex DNA-gold nanorods were characterized by transmission electron microscopy,fluorescence spectroscopy and UV absorption spectroscopy.The linearity range of the Pb（Ⅱ）found were within 100nmol·L^-1 and 150-1 000nmol·L^-1,with detection limit（3S/N）of 0.1nmol·L^-1.The sensor was used to analyze tap water samples,giving results in agreement with that obtained by ICP-AES.

关 键 词：金纳米棒 铅(Ⅱ) 荧光光谱法 四极子 

分 类 号：O657.34[理学—分析化学]