链霉菌Js-1T发酵条件及其对疣孢霉病的防治效果研究  

Optimization on Fermentation Conditions of Streptomyces Js-1T and Inhibition of the Fermented Liquid against Mycogone perniciosa

作　　者：李兵兵[1,2] 温志强[1] 

机构地区：[1]福建农林大学,福建福州350002 [2]江苏省淮安市疾病预防控制中心,江苏淮安223001

出　　处：《安徽农业科学》2015年第27期94-96,共3页Journal of Anhui Agricultural Sciences

基　　金：国家支撑计划项目(2013BAD16B03)

摘　　要：[目的]探讨链霉菌Js-1T的发酵条件及防治效果。[方法]通过单因素试验优化链霉菌菌株Js-1T防治疣孢霉病的发酵培养基和条件,测定其发酵液对疣孢霉菌(Mycogone perniciosa)的田间防治效果。[结果]Js-1T菌株的最佳培养基配方为:甘露糖30.0 g,KNO32.0 g,Mg SO41.5 g,K2HPO41.0 g,Na Cl 0.1 g,水1 000 ml;最佳发酵条件为:30℃,p H=8,250 ml三角瓶装液量为100 ml。发酵液对疣孢霉菌的田间防治效果显著,对Ⅰ类、Ⅱ类、Ⅲ类3个类群疣孢霉菌引起的疣孢霉病的防治效果分别达68.77%、83.84%、85.62%。[结论]链霉菌Js-1T在疣孢霉病防治上具有很好的应用前景。[ Objective ] The aim was to study fermentation conditions and control effect of Streptomyces Js-1T [ Method ] In order to increase the productivity of activities substance of strain Js-1T, the composition of fe.rmenting solution and fermenting conditions were optimized by single factor. The inhibition of its fermented liquid against Mycogone perniciosa was also tested. [ Result ] The optimum fermenting solution were Mannose 30.0 g, KNO3 2.0 g, MgSO4 1.5 g, K2HPO＊ 1.0 g, NaC1 0.1 g, and water 1 000 ml; the optimal fermenting conditions were tem- perature 30 ＂C, pH = 8, and 100 ml liquid medium in 250 ml flake. The inhibition of its fermented liquid against Mycogone perniciosa I , II and III were 68.77%, 83.84% and 85.62%, respectively. [ Conclusion] Streptomyces strain Js-1w had certain application prospect in control of Mycogone perniciosa.

关 键 词：链霉菌Js-1T 发酵优化 疣孢霉菌 生物防治 

分 类 号：S436.46[农业科学—农业昆虫与害虫防治]