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作 者:耿海东[1,2] 金洪梅[1] 张延君 张小林[1] 张佳洁[1] 李祥[1] 周长宇[1] 丁圆英 高光雨 王卓乐 乔丹[1] 李吟雪[1] 翟熙文 华育平[1] 张国钢[4] 柴洪亮[1]
机构地区:[1]东北林业大学野生动物资源学院,黑龙江哈尔滨150040 [2]国家林业局野生动物疫源疫病监测总站,辽宁沈阳110034 [3]中国野生动物保护协会秦皇岛野生动物救护中心,河北秦皇岛066100 [4]全国鸟类环志中心中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室,北京100091
出 处:《安徽农业科学》2015年第27期129-130,194,共3页Journal of Anhui Agricultural Sciences
基 金:林业公益性行业科研专项(201404404);国家林业局野生动物疫源疫病监测项目
摘 要:[目的]了解江西遂川地区野生鹭科鸟类携带禽流感和新城疫病毒的感染情况。[方法]于2014年10月在江西省遂川地区采集鹭科鸟类的肛拭子110份、咽拭子110份,采用SPF鸡胚进行病毒分离,通过血凝试验筛选出疑似阳性样品,然后再用RT-PCR进行鉴定。[结果]在110份被检样品中,除4份样品外,其余样品的血凝效价均为0。经RT-PCR检测,所有样品禽流感和新城疫病毒检测结果均呈阴性。这表明2014年秋季江西遂川地区野生鹭科鸟类发生禽流感和新城疫病毒感染的风险较小。[结论]该研究可为了解江西各地区野生水禽禽流感和新城疫的感染情况提供基础数据。[Objective] The research aimed to investigate the epidemiological characteristics of avian Influenza and newcastle disease virus of wild Ardeid birds in Suichuan Region of Jiangxi Province. [ Method] 110 oropharyngeat and 110 cloacal swabs of Ardeid birds were collected from Suichuan Region of Jiangxi Province in October of 2014. The viruses were separated by using SPF chicken embryos and suspected positive samples were screened out by hemagglutination test and identified by RT-PCR. [ Result] Among 110 detected samples,hemagglutination titer of other samples was 0 except 4 samples. The detection results of RT-PCR showed that the detection samples of avian Influenza and newcastle disease virus of all samples were negative. This indicated that the infection risk of avian Influenza and newcastle disease of Ardeid birds in Sui- chuan Region of Jiangxi Province in the summer of 2014 was small. [ Conclusion ] The research could provide basic data for understanding the infection situations of avian Influenza and newcastle disease of wild water birds in each region of Jiangxi Province.
分 类 号:S855[农业科学—临床兽医学]
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