宝天曼落叶阔叶林土壤细菌多样性  

Soil bacterial diversity in the Baotianman deciduous broad-leaved forest

作　　者：赵爱花[1,2] 杜晓军[1] 臧婧[1] 张守仁[1] 焦志华[3] 

机构地区：[1]中国科学院植物研究所植被与环境变化国家重点实验室,北京100093 [2]中国科学院大学,北京100049 [3]中国矿业大学(北京)化学与环境工程学院,北京100083

出　　处：《生物多样性》2015年第5期649-657,共9页Biodiversity Science

基　　金：国家自然科学基金(31270642;31370586)

摘　　要：土壤微生物在森林生态系统中起着重要作用。高通量测序方法的出现为进一步认识土壤微生物提供了契机。本文利用Illumina Miseq高通量测序技术对宝天曼森林土壤的细菌多样性进行了初步研究。结果显示:在31个采样点内,随着采样点增加,检测出不同分类水平的土壤细菌类群也在增多,当采样点达到31个时,检测出的土壤细菌类群达到45门163纲319目495科785属和42,632个OTU;31个土壤样品中所检测出的细菌类群平均有34.2门114.7纲215.2目323.7科446.6属5,924.7个OTU,其中门、纲、目分类水平上的优势类群(所占比例)分别为变形菌门(Proteobacteria)(38.30%)、α-变形菌纲(α-Proteobacteria)(18.08%)、根瘤菌目(Rhizobiales)(10.62%)。这些初步研究结果表明在一定程度上宝天曼森林土壤有较高的细菌多样性水平,为进一步认识森林土壤细菌多样性与植物多样性关系等奠定了基础。Soil microbes play an essential role in forest ecosystems. The development of the high-throughput sequencing method provides an invaluable opportunity to further understand soil microbial communities. In this study, we investigated soil bacterial diversity in the Baotianman forest using the Illumina Miseq plat- form. Results showed that in the 31 soil sampling points, the number of bacterial taxa detected at different classification levels increased with increasing number of sampling points. When all 31 samples were consid- ered, the number of relative bacterial groups included 45 phyla, 163 classes, 319 orders, 495 families, 785 genera and 42,632 OTUs. The mean values of relative bacterial taxa in the 31 samples were 34.2 phyla, 114.7 classes, 215.2 orders, 323.7 families, 446.6 genera, and 5,924.70TUs. At the classification level of phylum, class and order, the dominant groups were Proteobacteria （38.30%）, a-Proteobacteria （18.08%）, and Rhizobiales （10.62%）, respectively. These preliminary findings suggest that Baotianman forest soils have high levels of bacterial diversity to some degree and provide basic information and knowledge to further understand the relationship between soil bacterial diversity and plant diversity and other related scientific questions.

关 键 词：森林 土壤微生物 细菌多样性 高通量测序 变形菌门 α-变形菌纲 根瘤菌目 宝天曼国家级自然保护区 

分 类 号：S714.3[农业科学—林学]