海藻酸钙-超声辅助的多种雌激素增强生物降解  

Enhanced Biodegradation of Multiple Estrogens with Assistant of Calcium Alginate and Ultrasonic

作　　者：邱尤丽 张琛[1,2] 李鱼[1] 

机构地区：[1]华北电力大学资源与环境研究院,北京102206 [2]国家环境保护湖泊污染控制重点实验室,湖泊生态环境创新基地,北京100012

出　　处：《吉林大学学报（理学版）》2015年第5期1065-1072,共8页Journal of Jilin University:Science Edition

基　　金：吉林省财政专项基金吉林省水资源规划项目(批准号:2013-931)

摘　　要：通过向土壤样品溶液中添加海藻酸钙并辅以超声,提高多种雌激素(E1,E2,E3,EE2和BPA)的生物降解效率,采用中心复合设计及响应曲面优化方法确定最优降解条件,并通过生物降解性能关系(QSBR)分析雌激素理化性质对生物降解的影响.结果表明:在海藻酸钙增强生物降解体系中,5种雌激素同时降解的最优条件为超声3min,海藻酸钙的质量分数为5%,菌液添加量6mL;优化降解3d后,E1的降解率约为100%;7d后E3,BPA,EE2和E2的降解率分别约为52.65%,96%,96.90%和100%;雌激素的极性表面积(PSA)、辛醇-水分配系数(lg Kow)和表面张力为影响雌激素生物降解的主要参数;极性表面积和辛醇-水分配系数的交互作用、辛醇-水分配系数和表面张力的交互作用对雌激素的降解均表现为协同作用.We aimed at studying the effects of calcium alginate immobilized bacteria and ultrasonic assistance on the enhanced biodegradation of estrogens in E1,E2,E3,EE2 and BPA soil.The central composite design was utilized for the experiment design of ultrasonic time,the concentration of calcium alginate and the amount of immobilized bacteria.At the same time,the design was combined with the response surface method to optimize,to find the optimal degradation conditions of estrogens in soil and provide a theoretical foundation and basis on estrogens removal. The enhanced biodegradation mechanism of estrogens in soil by calcium alginate was analysed qualitatively.In the meantime,quantitative structure biodegradability relationship（QSBR）was utilized to analyze the effects of estrogenic physicochemical properties on the enhanced biodegradation mechanism,which was from the perspective of external and internal reasons.The results of our experiments and analysis show that under the addition of calcium alginate assisted with ultrasonic,the optimal conditions forthe biodegradation of five estrogens were as followed：ultrasonic time was 3 minutes,the mass concentration of calcium alginate was 5%,and the amount of bacteria liquid was 6 mL.Under the optimal conditions,if the system was degraded 3d,the biodegradation rate of E1 approached 100%;degraded 7d,the biodegradation rates of E3,BPA,EE2 and E2achieved 52.65%,96%,96.90% and100% respectively.The mechanism analysis also demonstrate that polar surface area（PSA）,hydrophobicity（represent by lg Kow）and surface tension of estrogens were the dominant parameters affecting estrogen biodegradation.In addition,the interaction of PSA and log Kow performed a synergistic effect on the degradation of estrogens,the interaction of lg Kow and surface tension also performed a synergistic effect on the degradation of estrogens.Therefore,it is clear that calcium alginate with ultrasonic assistance can significantly improve the biodegradation rates of estrogens in soil.

关 键 词：雌激素 土壤 海藻酸钙 生物降解性能关系 生物降解机理 

分 类 号：X53[环境科学与工程—环境工程]