抗水稻白叶枯病新基因Xa-25(t)的RAPD分析  

RAPD Analysis of A New Resistance Gene Xa-25(t) to Rice Bacterial Blight

作　　者：高东迎[1,2] 向阳海[3] 孙立华[4] 陆作楣[1] 

机构地区：[1]南京农业大学农学系 [2]江苏省农业科学院农业生物遗传生理研究所,江苏南京210014 [3]江苏省农业科学院农业生物遗传生理研究所1江苏南京210014 [4]江苏省农业科学院农业生物遗传生理研究所

出　　处：《中国水稻科学》2002年第2期179-181,共3页Chinese Journal of Rice Science

基　　金：国家"九五"重点攻关资助项目 (96-C0 1-0 1-0 1)

摘　　要：利用 RAPD标记对从体细胞突变体 HX- 3中鉴定出的抗水稻白叶枯病新基因 Xa- 2 5 (t)进行了研究 ,在 30 6个随机引物中 ,两个引物 S12 6 9和 S132 7在亲本和抗感池 (均由 12个高抗或高感植株组成 )之间表现多态性。用这两个引物对龙特甫 A和 HX- 3的 F2 群体的 114个单株进行连锁分析 ,表明 S12 6 9和 S132 7与 Xa- 2 5 (t)的连锁距离分别为 5 .3和 2 3.7c M,且位于 Xa- 2 5 (t)的两侧。Xa 25(t), a new resistance gene to bacterial blight identified from the somaclonal mutant HX 3, was studied by using RAPD marker. Out of the 306 random primers tested,two were found to be polymorphic between the parents and the resistant pool and susceptible pool(which were composed of 12 highly resistant plants and highly susceptible plants,respectively). With the two primers, 114 individuals from the F 2 population of Longtefu A/HX 3 were analyzed for linkage relationship. Results indicated that S1269 and S1327 were located on the opposite sides of Xa 25(t), with the distances of 5 3 cM and 23.7 cM respectively.

关 键 词：RAPD分析 水稻 白叶枯病 体细胞突变体 抗病基因 随机扩增多态性DNA 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]