8-羟基喹啉荧光法测定口服液中微量锌  

Determination of trace oral zinc by 8-hydroxyquinoline fluorescence spectrophotometry

作　　者：李玉红[1] 孟小华[1] 杨振[1] 赵琴[1] 

机构地区：[1]咸阳师范学院化学与化工学院,陕西咸阳712000

出　　处：《化学工程师》2015年第9期23-24,22,共3页Chemical Engineer

基　　金：咸阳师范学院自然科学基金资助课题(13XSYK019)

摘　　要：在p H值为5.0的HAc-Na Ac缓冲介质中,8-羟基喹啉与锌可发生反应形成二元络合物,络合物在497nm处产生较强的荧光峰,锌质量浓度在0~5.0μg·m L-1范围内与荧光强度呈良好的线性关系,方法检测限为0.099μg·m L-1,加标回收率在96.5%~104.5%之间,在20~40℃范围内,ln F与1/T呈线性关系,表观活化能Ea为61.55k J·mol-1,用于口服液中锌质量浓度的测定,结果符合要求。In the Na Ac-HAc buffer solution with p H of 5.0, 8-hydroxyl quinoline and Zinc reaction to form stable binary-complex,which had a strong fluorescence peak at 497 nm. The Zinc concentration ranges from 0 to 5.0μg·m L-1, the fluorescence intensity displays a good linear relationship with Zinc concentration. Under the following preconditions： the detection limit of 0.099μg·m L-1, the recovery of standard addition of 96.5% ~104.5%, In the range of 20~40℃, the ln F and 1/T is linear, the apparent activation energy Ea is 61.55 k J·mol-1, for the determination of the mass concentration of zinc in oral, the results meet the requirements, and it is satisfied with people.

关 键 词：荧光光度法 口服液 锌 

分 类 号：O657[理学—分析化学]