结核分枝杆菌感染对小鼠树突状细胞功能的影响  

Effects of Mycobacterium tuberculosis on dendritic cells function

作　　者：金齐力[1,2] 孙悝[1] 吕小艳[1] 韦莉[2,3] 

机构地区：[1]蚌埠医学院第二附属医院检验科,安徽蚌埠233040 [2]蚌埠医学院感染与免疫安徽省重点实验室,安徽蚌埠233030 [3]蚌埠医学院病原生物学教研室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2015年第9期1145-1147,1151,共4页Journal of Bengbu Medical College

基　　金：安徽省自然科学基金项目(1508085QH175);安徽省高校省级自然科学研究项目(KJ2013B142)

摘　　要：目的：探讨结核分枝杆菌（ Mycobacterium tuberculosis,Mtb）感染对小鼠骨髓来源的树突状细胞（ DC）功能的影响。方法：将DC分为4组,即脂多糖（ LPS）感染组、Mtb感染组、灭活Mtb感染组和正常细胞对照组。以LPS、Mtb及灭活的Mtb与小鼠骨髓来源的DC建立小鼠体外感染模型,用酶联免疫吸附法测定白细胞介素（ IL）-6、IL-12及肿瘤坏死因子-α的表达；流式细胞术检测DC表面主要组织相容性复合体Ⅱ类分子、CD40、CD80和CD86的表达。结果：每只小鼠的股骨骨髓可扩增获得5×10^6~1×10^7个具有典型细胞形态的DC,纯度达85%以上；与对照组比较,流式细胞术结果显示LPS、Mtb和灭活Mtb感染组均能促进DC表面分子的表达（P〈0.01）。 Mtb感染组DC表面分子上调显著低于LPS感染组与灭活Mtb感染组（P〈0.01）；LPS、Mtb或灭活Mtb作用后,DC的IL-6、IL-12和肿瘤坏死因子-α分泌量显著增加（P〈0.01）,Mtb感染组DC的细胞因子分泌量显著低于LPS感染组与灭活Mtb感染组（P〈0.01）。结论：Mtb活菌可干扰DC的细胞因子分泌,抑制DC成熟,从而削弱其抗原递呈的功能,影响抗原特异性细胞的免疫活化。Objective：To investigate the effect of Mycobacterium tuberculosis（Mtb） on the function of mouse bone marrow derived dendritic cells（DC）. Methods：DC were divided into 4 groups,lipopolysaccharide（LPS）-treated group,Mtb-treated group,inactivated Mtb-treated group and control group. Cytokines including interleukin（IL）-6,IL-12 and tumor necrosis factor α secretion of infected cells were examined by ELISA method. And the expression of surface molecules of infected DC including major histocompatibility complex class Ⅱ,CD40,CD80 and CD86 were detected by flow cytometry. Results：About 5 × 10^6 to 1 × 10^7 DC were obtained from bone marrow of one mouse,and the purity of DC was more than 85%. The cells had the typical morphology of DC. The expressions of major histocompatibility complex classⅡ,CD40,CD80 and CD86 on stimulated DC increased in LPS-treated group,Mtb-treated group, inactivated Mtb-treated group in contrast to control group;while the percentage of the upregulation of surface molecules in Mtb-treated DC was significantly lower than that of LPS or inactivated Mtb-treated DC. The cytokine levels in all groups significantly increased,and IL-6,IL-12 and tumor necrosis factor α secretions of DC infected by Mtb were significantly lower than those of LPS or inactivated Mtb-treated DC. Conclusions：Mtb can decrease the antigen presenting function through interfering the secretion of cytokines and inhibiting maturation of DC,and consequently suppress DC and T cell activation and result in the more serious damage of host.

关 键 词：结核分枝杆菌 树突状细胞 免疫应答 小鼠 

分 类 号：R378.911[医药卫生—病原生物学]