牛蒡苷元对大鼠单个心室肌细胞电生理的影响  

Effects of arctigenin on the electrophysiology of single ventricular myocytes in rats

作　　者：陈海宁[1] 冯强[2] 单宏丽[2] 刘影[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生学教研室 [2]哈尔滨医科大学药学院药理学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2015年第4期292-295,共4页Journal of Harbin Medical University

基　　金：黑龙江省教育厅项目(11551243);市科委项目(2010RFQXS068)

摘　　要：目的研究牛蒡苷元(arctigenin,ARC)对大鼠心肌细胞上的瞬时外向钾电流(Ito)、内向整流钾电流(IK1)、L-型钙电流(ICa-L)以及动作电位时程的影响。方法酶解法分离大鼠单个心室肌细胞,应用全细胞膜片钳技术研究ARC对大鼠单个心室肌细胞电生理的影响。结果 100μmol/L、1 mmol/L和10mmol/L ARC呈剂量依赖性延长心肌细胞动作电位时程。100μmol/L、1 mmol/L和10 mmol/L的ARC可显著抑制ICa-L,但对Ito和IK1无显著影响。结论 ARC可通过抑制ICa-L而延长动作电位时程,从而具有抗心律失常作用。Objective To study the effects of arctigenin （ARC） on transient outward potassium current （Ito）, inward rectifier potassium current （IK1） , L-type calcium current and action potential duration （APD） of the single ventricular myocytes in rats. Methods Enzymatic dis- sociation was used to isolate single ventricular myocytes in rats. Whole cell patch clamp technique was used to investigate the influences of arctigenin on electrophysiology of rats single ventricular myocytes. Results 100μmoL/L, 1 mmol/L and 10 mmol/L ARC prolonged APD in a concentration-dependent manner. 100μmol/L, 1 mmoL/L and 10 mmol/L ARC significantly inhibited the L-type calcium current, had no impacts on Ito and IKi. Conclusion ARC has the effect of anti-arrhythmia by inhibiting L-type calcium current and prolonging action potential duration.

关 键 词：牛蒡苷元 心肌细胞 动作电位 钙电流 钾电流 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]