日本七鳃鳗(Lampetra japonica)髓细胞cDNA文库构建及EST序列分析  

The construction of myeloid-cells cDNA library and bioinformatics analysis of Lampetra japonica

作　　者：李庆伟[1,2] 梁宏芳 潘雄[1,2] 逄越[1,2] 朱婷[1,2] 徐扬[1,2] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081 [2]辽宁师范大学七鳃鳗研究中心,辽宁大连116081

出　　处：《辽宁师范大学学报（自然科学版）》2015年第3期375-380,共6页Journal of Liaoning Normal University:Natural Science Edition

基　　金：国家重大基础研究发展计划(973)课题(2013CB835304);全国海洋公益项目(20130501);国家自然科学基金项目(31170353;31202020);大连市科技计划项目(2013E11SF056)

摘　　要：选取日本七鳃鳗(Lampetra japonica)神经轴体中分离出的髓细胞,采用SMART技术成功构建七鳃鳗髓细胞cDNA文库,并随机挑选部分克隆子进行测序及生物信息学分析,对其质量进行了检测.检验结果显示,本次实验所构建的cDNA文库滴度>2.8×105 pfu/mL,符合大规模测序的标准.运用Blast2GO软件进行生物信息学比对,发现5 749个转录本在NCBI数据库中找到同源或相似序列.其中分别有39.52%、30.30%和30.18%的转录本承担生物学途径、分子功能和细胞组件等功能.EST序列的研究是目前为止获得新功能基因最有效的手段,日本七鳃鳗髓细胞cDNA文库的构建成功,将为下一步在七鳃鳗髓细胞中寻找具有研究价值的新功能基因指明方向.To achieve the genetic information on lampry Li-body and to explore the novel genes for anti-cancer,a cDNA library has been constructed from myeloid cells of lamprey.The results showed that original library＇s titer was 2.8×10^5 pfu/mL.Building of the library has a good quality and meets the requirements of large-scale sequencing.5749 transcripts has similar sequences published in NCBI databases.The functional ESTs with the Blast2 GO software showed that 39.52%,30.30% and30.18% of the transcripts were involved in biological pathways,molecular function,and cellular components.ESTs research,which is effective means to obtain functional genes,Japanese lamprey myeloid cells cDNA library of successful build for the future screening of new genes related to fight cancer,laid the foundation for the further system to study the mechanism against cancer.

关 键 词：日本七鳃鳗 神经轴体 髓细胞 CDNA文库 表达序列标签 

分 类 号：Q291[生物学—细胞生物学]