运动强度诱导2型糖尿病大鼠肌IRS-2介导活动的研究  

Exercise intensity induced the transduction of IRS-2 in skeletalmuscle of T2DM rats

作　　者：王耀光[1] 宫华[1] 金爱晶[2] 郭仁勇[3] 

机构地区：[1]辽宁师范大学体育学院,辽宁大连116029 [2]澳大利亚联邦大学教育与人文学院 [3]辽宁师范大学生命科学学院,辽宁大连116081

出　　处：《辽宁师范大学学报（自然科学版）》2015年第3期421-426,共6页Journal of Liaoning Normal University:Natural Science Edition

基　　金：国家体育总局武术研究院武术研究项目(WSH2013B002);辽宁省教育厅科学技术研究项目(20060474)

摘　　要：为了观察不同运动强度对SD大鼠骨骼肌细胞胰岛素受体底物-2(IRS-2)蛋白含量及磷酸化的影响,先建立2型糖尿病大鼠模型,建模后将SD大鼠分为6组:对照组及糖尿病对照组(n=10)和糖尿病运动组(15 m/min组(n=9),20 m/min组(n=10),25m/min组(n=10),30m/min组(n=9)).运动组跑台连续运动6周,每天50min.用Western blot(免疫印迹法)检测鼠肌细胞IRS-2蛋白含量及磷酸化程度.实验结果发现,与糖尿病对照组相比,15、20、25、30m/min组鼠肌IRS-2蛋白含量分别升高17%(P<0.05)、24.4%、23.4%和21.3%(P<0.01);15、20、25、30m/min组鼠肌IRS-2磷酸化分别升高34.7%、37.2%、40.2%和38.8%(P<0.01).实验结果提示,不同运动强度对IRS-2含量产生显著影响,而对IRS-2磷酸化程度不产生明显影响.The study aim to explore effects of different intensity exercise on IRS-2protein expression rats with diabetes.Sixty SD rats were divided into six groups：normal control group（NC）,diabetes mellitus group（DMC）,15m/min group,20m/min group,25 m/min group and 30 m/min group.The exercise groups did the running exercise each day for 50 min,lasting for six weeks.The contents of IRS-2protein were measured by Western bolt analysis.The contents of IRS-2protein in exercise groups enhanced up（P〈0.01）compared with the DMC group;25and 30m/min groups has the significant difference（P〈0.01 and P〈0.05）compared with 15 and 20m/min group,respectively.The tyrosine phosphorylation of IRS-2protein in exercise groups enhanced up（P〈0.01）compared with the DMC group,but still in a lower level compared with the NC group.20、25and30m/min groups have the significant differences（P〈0.01 and P〈0.05）compared with 15m/min group,respectively.20 and 25m/min groups have the significant differences（P〈0.05）compared with 30m/min group,respectively.The results showed that the different intensity exercise can affect the contents of IRS-2protein and the tyrosine phosphorylation of IRS-2protein in skeletal muscle in T2 DM rats.

关 键 词：运动强度 糖尿病大鼠 骨骼肌 IRS-2 

分 类 号：G804.8[文化科学—运动人体科学]