子宫内膜异位症大鼠内膜窗口期模型的建立  

Establishment of the endometriosis rat model in intimal window period

作　　者：曹芳[1] 黄晓阳[1] 葛素梅[1] 许科[1] 陈莉[1] 

机构地区：[1]南京医科大学附属常州市妇幼保健院生殖中心,213000

出　　处：《重庆医学》2015年第27期3805-3807,共3页Chongqing medicine

基　　金：南京医科大学科技发展基金重点项目(2013NJMU213)

摘　　要：目的研究子宫内膜异位症(EMs)患者窗口期的子宫内膜容受性,构建EMs大鼠内膜窗口期动物模型。方法取性成熟SD大鼠22只,通过手术将SD大鼠自体子宫内膜组织移植至同侧腹壁及对侧子宫系膜上,建立诱发型EMs大鼠动物模型。术后4周随机选取2只大鼠解剖观察异位内膜的生长情况,确定造模成功后,将EMs模型大鼠与雄鼠同笼交配,次日观察阴栓,妊娠第5天剖腹观察20只模型大鼠异位内膜的存活情况,并对在位子宫内膜及异位内膜进行组织形态学观察。结果 20只妊娠第5天大鼠均有明显的异位病灶,在腹壁及系膜上均呈小包块样生长,且与周围组织有不同程度的粘连,光镜下见异位子宫内膜形态具有正常子宫内膜基本组织结构,EMs大鼠造模成功;所有EMs模型大鼠与雄鼠同笼交配次日均见阴栓,妊娠第5天大鼠非手术侧子宫腔内不同程度积液,在位及异位内膜均处于分泌期。结论通过自体子宫内膜移植法成功建立了EMs大鼠模型;EMs模型大鼠子宫内膜处于胚胎着床窗口期,为研究EMs子宫内膜容受性提供了动物模型。Objective To research the endometrial receptivity by established the endometriosis rat model in intimal window period.Methods The autologous endometrial tissues of 22 sexually mature SD rats were transplanted into the homonymy abdominal wall and offside uterine mesometrium.After four weeks,we randomly dissected two rats and observed the growth of etopic endometrium.The EMs rats were mated with male rats in the same cage and vaginal plug was observed in the next day.The histomorphology of eutopic and ectopic endometrial was observed in the fifth day of pregnancy.Results Ectopic endometrium was grown in the abdominal wall and mesometrium,which formed a small bulge cystic mass in 20 rats.All foci was showed adhesion to the surrounding tissues in a different extent.In the light microscope,the morphology of ectopic endometrial was as the same as nomal endometrium.The establishment of the endometriosis rat mode is successful.Vaginal plug was positive the next day.Nonoperative side uterine was full of fluid in the fifth day of pregnancy.Eutopic and ectopic endometrium were in the secretory phase..Conclusion It is a successful method to establish a rat model of endometriosis by autotransplantion of endometrium.The endometrium of EMs model rats is in embryo implantation window period,and it provides a convenient model for studying endometrial receptivity.

关 键 词：子宫内膜异位症 模型 动物 大鼠 

分 类 号：R711.71[医药卫生—妇产科学]