推拿对坐骨神经损伤大鼠背根神经节降钙素基因相关肽表达的影响  

Effects of Massage on the Expression of Calcitonin Gene-Related Peptide in Dorsal Root Ganglion of Chronic Constriction Injury Rats

作　　者：冼思彤 于天源[1] 潘璠[1] 鲁梦倩[1] 吴剑聪[1] 高玉峰[1] 张林峰[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中医学报》2015年第9期1311-1314,共4页Acta Chinese Medicine

基　　金：国家自然科学基金资助项目(81373759);北京市自然基金资助项目(7142097);博士点基金资助项目(20130013110016)

摘　　要：目的:观察推拿对坐骨神经损伤后背根神经节神经元降钙素基因相关肽(calcitonin gene-related peptide,CGRP)表达的影响。方法:48只大鼠随机分为正常组、假手术组、模型组、模型对照组、推拿组,采用坐骨神经损伤(chronic constriction injury,SNI)模型,以按摩推拿手法模拟仪进行推拿手法干预,观察各组大鼠光热耐痛阈、痛敏分数变化及L3-5节段右侧背根神经节CGRP的表达情况。结果:造模7 d后,与同期正常组比较,模型组大鼠的耐痛阈值及痛敏分数均明显升高(P<0.01);模型组CGRP在背根神经节的表达升高(P<0.01)。在推拿治疗20次之后,与同期模型组比较,推拿组耐痛阈值及痛敏分数明显降低(P<0.05);与同期模型组和模型对照组比较,推拿组CGRP在背根神经节的表达明显升高(P<0.01)。结论:推拿可以提高周围神经损伤大鼠背根神经节内CGRP的表达,参与神经损伤修复过程,最终改善周围神经损伤大鼠的感觉功能。Objective：To observe the effect of massage on calcitonin gene-related peptide（CGRP）expression of the dorsal root ganglion neurons after sciatic nerve injury（SNI）. Methods：48 rats were randomly divided into normal group,control group,model group,model control group,massage group,SNI model. Used manipulation maneuver simulation for intervention. Observed pain threshold and pain tol-erance sensitivity scores of field change and L3 - 5 section on the right side of CGRP expression in dorsal root ganglia. Results：After building for 7 days,compared with normal group,the pain tolerance threshold,tolerance sensitivity scores and CGRP expression in dorsal root ganglia of model group rats were significantly higher（P ﹤ 0. 01）. After massage therapy 20 times,compared with the model group, the pain tolerance threshold and tolerance sensitivity scores of massage group were significantly decreased（P ﹤ 0. 05）. Compared with model group and model control group,the CGRP expression in dorsal root ganglia of massage group was increased significantly（P ﹤0. 01）. Conclusion：Massage can improve the CGRP expression of the dorsal root ganglion neurons,participate in neurological damage repair process so as to eventually improve the function of the feeling of peripheral nerve injury in rats.

关 键 词：推拿 坐骨神经损伤 降钙素基因相关肽 背根神经节 大鼠 

分 类 号：R285.5[医药卫生—中药学]