红色中缢虫赤潮中浮游植物细胞碱性磷酸酶的研究  

The study on alkaline phosphatase activity( APA) of phytoplanktons in the bloom of Mesodinium rubrum based on ELF@97

作　　者：庞勇[1,2] 聂瑞[2,3] 欧林坚[2] 吕颂辉[2] 

机构地区：[1]海南省海洋与渔业科学院,海南海口570125 [2]暨南大学赤潮与海洋生物学研究中心,广东广州510632 [3]海南省海洋监测预报中心,海南海口570206

出　　处：《海洋环境科学》2015年第5期736-742,共7页Marine Environmental Science

基　　金：国家自然科学基金(U0733006);国家973研究计划项目(2010CB428702);国家科技部海洋公益性专项(200805015)

摘　　要：2009年12月14日,在对球形棕囊藻Phaeocystis globosa赤潮的跟踪调查过程中发现一次红色中缢虫赤潮,设置3个站位对红色中缢虫赤潮进行调查监测。利用酶标记荧光(enzyme labeled fluorescence,ELF@97)方法,对浮游植物碱性磷酸酶进行标记,研究红色中缢虫赤潮发生时,单个浮游植物碱性磷酸酶活性特征。得出以下结论:(1)浮游植物碱性磷酸酶总体的标记率达51%,其中甲藻的碱性磷酸酶具有很强的表达,标记率超过99%;硅藻的碱性磷酸酶表达很弱,标记率仅为3.4%。(2)甲藻比硅藻很容易被诱导产生碱性磷酸酶。(3)浮游植物在与红色中缢虫营养盐竞争中处于劣势。A bloom of Mesodinium rubrum was found in the investigation of the bloom of Phaeocystis globosa on Decem- ber 14th,2009. Then,three sampling stations were set up for monitoring the bloom of Mesodinium rubrum. During the, bloom of Mesodinium rubrum,the alkaline phosphatase activity of a single phytoplankton was characterized using the methodology of enzyme-labeled fluorescence. It was found that ： （ 1 ） the overall labeled rate of alkaline phosphatase ac- tivity in phytoplankton was 51%, in which dinoflagellate had a strong expression of alkaline phosphatase with a labeled rate higher than 99% ; while diatoms had a very weak expression of alkaline phosphatase with a labeled rate of only 3.4%; （2） dinoflagellate was easier to be induced to produce alkaline phosphatase with respect to diatoms; （3） phytoplankton was at a disadvantage in competing for nutrients, compared to Mesodinium rubrum.

关 键 词：浮游植物 ELF 碱性磷酸酶 

分 类 号：Q178.53[生物学—水生生物学]