复杂生物样品中马来酸依那普利的快速分析新方法  

A Method for Fast Determination of Enalapril Maleate in Complex Biological Matrices

作　　者：杨天鸣[1] 黄国贞 付海燕[1] 周蓉[1] 李鹤东 姜杜 

机构地区：[1]中南民族大学药学院,湖北武汉430074

出　　处：《化学与生物工程》2015年第9期65-69,共5页Chemistry & Bioengineering

基　　金：国家"十二五"科技支撑计划资助项目(2012BAI27B00);国家自然科学基金资助项目(21205145);中央高校基本科研业务费专项资金资助项目(CZZ10005;CZY12028;CZY13019)

摘　　要：建立了复杂生物基质中马来酸依那普利的快速检测新方法。运用高效液相色谱-二极管阵列(HPLC-DAD)检测技术结合自加权交替三线性分解(SWATLD)二阶校正算法直接快速测定血浆、唾液和尿液生物基质中马来酸依那普利的含量,获得血浆、唾液和尿液3种生物基质中马来酸依那普利的平均回收率分别为(98.0±3.7)%、(98.1±6.0)%和(100.8±3.4)%。通过分析品质因子,包括灵敏度(SEN)、选择性(SEL)、检测限(LOD)、检测量(LOQ)和预测均方差(RMSEP)评估了该方法的性能,利用t-检验对结果进行了校验。结果表明,该方法具有的"二阶优势"可在不同生物基质样中内源化学物质干扰共存下,有效分辨和定量分析马来酸依那普利,结果准确可靠。A method for fast determination of enalapril maleate in complex biological matrices was devel-oped.The content of enalapril maleate in plasma,saliva and urine matrices were directly and rapidly determina-ted by HPLC-DAD combined with the second-order correction algorithm of SWATLD.The average recovery of enalapril maleate in three biological matrices of plasma,saliva and urine were （98.0±3.7）%,（98.1 ±6.0）%and （100.8±3.4）%,respectively.The performance of the method was evaluated by analyzing the quality fac-tors,including sensitivity（SEN）,selectivity（SEL）,limit of detection（LOD）,limit of quantitation（LOQ）and RMSEP.t-Test was used to check the results.This method had the “second-order advantage”.Enalapril mal-eate was distinguished efficiently and analyzed quantitatively in the presence of different biological matrixs with endogenous substances,and the analytical results were accurate and reliable.

关 键 词：马来酸依那普利 HPLC-DAD SWATLD 血浆 唾液 尿液 

分 类 号：O657.72[理学—分析化学]