cornulin在宫颈病变中的表达及其差异  

The expression and its difference of cornulin in cervical lesions

作　　者：刁雯静[1] 孙红[1] 郭奇桑[1] 王丽[1] 陶祥[1] 隋龙[1] 

机构地区：[1]复旦大学附属妇产科医院检验科,上海200011

出　　处：《检验医学》2015年第9期898-902,共5页Laboratory Medicine

基　　金：中国癌症基金会资助项目(E12012001)

摘　　要：目的了解宫颈炎、宫颈癌前病变及宫颈癌组织中cornulin的表达情况,初步评价cornulin与宫颈癌变的相关性。方法收集各个级别的宫颈癌前病变及可疑宫颈癌患者行诊断性或治疗性经宫颈环形电切割术(LEEP)后的石蜡包埋组织标本131例,其中宫颈低级别鳞状上皮内病变(LSIL)39例、宫颈高级别鳞状上皮内病变(HSIL)49例、宫颈浸润癌(ICC)43例,以38例宫颈炎患者作为对照组。所有标本经兔抗人cornulin多克隆抗体免疫组化链霉菌抗生物素蛋白-过氧化物酶连结(SP)法显示并评分。在行宫颈LEEP术之前取其宫颈脱落细胞,宫颈炎患者经阴道镜下活检病理诊断为慢性宫颈炎后取其宫颈脱落细胞,采用聚合酶链反应(PCR)测定cornulin基因的mRNA表达。结果免疫组化结果显示,除宫颈炎组与宫颈LSIL组之间无差异(P=0.148)外,其余各组间cornulin蛋白表达差异均有统计学意义(P均=0.000);随着宫颈病变级别的逐渐升高,cornulin蛋白表达逐渐下降,至ICC时表达明显降低;cornulin mRNA的表达与其蛋白表达一致。以cornulin弱阳性(+)或阴性(-)作为筛查阳性指标,检出宫颈HSIL以上病变的敏感性为70.7%,特异性为85.7%;检出宫颈LSIL以上病变的敏感性为56.5%,特异性为94.7%。结论 cornulin有望成为检测宫颈病变程度的标记物之一。Objective To study the cornulin expressions in cervicitis tissue, cervical intraepithelial neoplasia tissue and cervical cancer tissue, and to evaluate preliminarily the correlation of cornulin with cervical intraepithelial neoplasia tissue.Methods A total of 131 paraffin-embedded tissue samples were collected from cervical intraepithelial neoplasia and suspicious cervical cancer patients undergoing loop electrosurgical excision procedure （ LEEP ） for diagnostics or treatment.They included 39 patients with low-grade squamous intraepithelial lesion （LSIL）, 49 patients with high-grade squamous intraepithelial lesion（ HSIL） , 43 patients with invasive cervical cancer（ ICC） , and 38 patients with cervicitis were enrolled as control group.Immunohistochemistry [streptavidin-peroxidase （SP）] using a rabbit polyclonal antibody against human cornulin was applied in all samples.Polymerase chain reaction （ PCR） was used for the determination of cornulin mRNA expression in all 131 patients′cervical thinprep cytologic test samples prior to cervical LEEP procedure and control group′s cervical thinprep cytologic test samples after histopathology diagnosis by colposcopy.Results Immunohistochemistry showed that the expression levels of cornulin were significantly different with the other groups （P=0.000）except that between LSIL and control group（P=0.148）.With the severity degree of cervical disease increasing, the expression of cornulin decreased, and it decreased significantly in ICC.The expression of cornulin mRNA was consistent with the results of cornulin expression level.If taking cornulin weakly positive（＋） or negative （-） as the cut-off for screening cervical HSIL or higher, the sensitivity was 70.7%, and the specificity was 85.7%.And for screening cervial LSIL or higher, the sensitivity was 56.5%, and the specificity was 94.7%. Conclusions Cornulin might be one of the useful markers for cervical carcinogenesis.

关 键 词：cornulin 宫颈癌 免疫组化 

分 类 号：R446.1[医药卫生—诊断学]