槐耳浸膏联合化疗药物对人胃癌MGC803的体外抑制作用  

Inhibition Effect of Extractum Robinia-living trametes Combined with Chemotherapy on Proliferation of Gastric Cancer Cell MGC803 in Vitro

作　　者：周玲[1] 乐涵波[2] 

机构地区：[1]浙江省舟山医院药剂科,浙江舟山316021 [2]浙江省舟山医院胸外科,浙江舟山316021

出　　处：《中国药学杂志》2015年第18期1607-1612,共6页Chinese Pharmaceutical Journal

摘　　要：目的筛选出槐耳浸膏与化疗药物的最佳组合并探讨槐耳浸膏和化疗药物5-氟尿嘧啶诱导人胃癌MGC803细胞凋亡的机制。方法以不同浓度槐耳浸膏和化疗药物(5-氟尿嘧啶,紫杉醇)作用于体外培养的人胃癌细胞MGC803,通过MTT法检测槐耳浸膏对MGC803细胞增殖的作用,倒置显微镜观察其形态的变化;将0.2 mg·m L-1的槐耳浸膏分别与5-FU(2.5μg·m L-1)或紫杉醇(0.5μg·m L-1)联合处理细胞,比较存活率。流式细胞术检测0.2 mg·m L-1槐耳浸膏联合5-FU(2.5μg·m L-1)干预MGC803细胞24、48 h后细胞的凋亡情况;Western Blot法检测0.2 mg·m L-1槐耳浸膏联合5-FU(2.5μg·m L-1)干预MGC803细胞24、48 h后细胞的P53、T-Akt和P-Akt蛋白的表达。结果槐耳浸膏及化疗药物(5-氟尿嘧啶,紫杉醇)均能降低MGC803细胞存活率,并呈时间和剂量依赖性;倒置显微镜下可见细胞胞体缩小、核碎裂,且呈浓度与时间依赖性(P<0.01)。相较于单独应用化疗药物,联合用药能够显著降低细胞的生存率。槐耳浸膏联合5-FU联合给药上调人胃癌细胞MGC803 T-Akt及P53蛋白表达量,同时下调P-Akt的表达量。结论槐耳浸膏联合5-FU在体外诱导胃癌细胞MGC803凋亡作用可能是通过抑制PI3K/Akt信号通路来实现的。联合使用槐耳浸膏和化疗药物可能会更有效发挥槐耳抗肿瘤增殖的作用。OBJECTIVE To screen the best combination of extractum of Robinia-living trametes and chemotherapy and investigate the action mechanism of Robinia-living trametes against the apoptosis of human gastric cancer cell MGC803. METHODS MGC803 Cells were treated with different concentrations of Robinia-living trametes and chemotherapy drugs（ 5-Fu and paclitaxel） in vitro. The inhibitory rate of cells was measured by MTT assay. Morphological changes were observed with inverted microscope. The apoptosis rate of MGC803 cells which were treated with combination of Robinia-living trametes（ 0. 2 mg·m L^- 1） and 5-Fu（ 2. 5 μg·m L^- 1） was detected by FCM. The protein expression of P53 and p-Akt in MGC803 cells which were treated with combination of Robinia-living trametes（ 0. 2 mg·m L^- 1） and 5-Fu（ 2. 5 μg·m L^- 1） was detected by Western blot. RESULTS The viability of MGC803 cells was reduced by Robinia-living trametes and chemotherapy drugs（ 5-Fu and paclitaxel） in a concentration-and time-dependent manner（ P〈0. 01）. Under reverse microscopy,cell body shrinking,nuclear pyrosis,and nuclear fragmentation were observed. The higher concentration,the longer treatment time,the more cells died. Compared with monotherapy,the combination of Robinia-living trametes and chemotherapy could reduce the survival rate of MGC803 cells. The protein expressions of P53 in MGC803 cells treated with combination of drugs was up-regulated,while that of P-Akt was down-regulated. CONCLUSION The apoptosis of MGC803 cells in vitro may be induced by the inhibitory effect of the combination of Robinia-living trametes and 5-Fu on PI3 K / Akt signaling pathway. Combination therapy of Robinia-living trametes and 5-Fu is potentially more effective in inhibition of tumor cells than monotherapy of Robinia-living trametes.

关 键 词：槐耳 紫杉醇 人胃癌细胞MGC803 细胞凋亡 P53蛋白 P-Akt蛋白 T-Akt蛋白 

分 类 号：R969[医药卫生—药理学]