小麦抗叶锈近等基因系TcLr38的SRAP-SSR(eSSR)分析  

Analysis of wheat leaf rust resistant near isogenic lines TcLr38 by SRAP-SSR(eSSR)

作　　者：闫红飞[1] 李令蕊 康健[1] 刘春燕[1,3] 王向东 刘大群[1] 

机构地区：[1]河北农业大学植物病理系/河北省农作物病虫害生物防治工程技术研究中心,河北保定071001 [2]河北省植保植检站,河北石家庄050011 [3]河北北方学院动物科技学院,河北张家口075131 [4]唐山市农科院,河北唐山063000

出　　处：《河北农业大学学报》2015年第5期6-10,共5页Journal of Hebei Agricultural University

基　　金：国家重点基础研究发展计划(2013CB127700);河北省自然基金(C2015204105);唐山市科技局项目(13130246z)

摘　　要：小麦抗叶锈基因Lr38在我国高抗叶锈病,直到目前该基因的分子标记开发较少,开发其分子标记对于该基因的研究与利用具有重要意义。利用594对SRAP-SSR(eSSR)引物组合对以Thatcher为背景小麦抗叶锈近等基因系材料TcLr38和Thatcher进行PCR扩增。引物组合ARBI8-Xcwem8R和ARBI2-Xgwm497F在TcLr38中分别扩增出300和400bp多态性条带。利用47个小麦抗叶锈近等基因系进一步检测,引物ARBI8-Xcwem8R仅在TcLr38中扩增出特异条带。经TcLr38×Thatcher F2代分离群体验证,该标记与Lr38遗传距离较远。对该片段克隆测序,片段长度为277bp,BLAST比对与GenBank中所收录序列无相似性,该片段为与Lr38相关的新的序列。Wheat leaf rust resistant gene L r38 is highly resistant to leaf rust in China .Until now ,the molecular markers of L r38 are less developed .So ,the development of molecular markers of L r38 is important for the research and utilization of this gene .In this study ,a to‐tal of 594 pairs of SRAP‐SSR （eSSR） primers were used to amplify the TcLr38 and back‐ground material Thatcher .Two primers ARBI8‐Xcwem8R and ARBI2‐Xgwm497F amplified polymorphic bands ,the size about 300 bp and 400 bp ,respectively .In addition ,47 w heat leaf;rust resistant near isogenic lines （NILs） were detected by these markers ,primer ARBI8‐Xc‐wem8R amplified a specific band only in TcLr38 .The F2 segregating population screening re‐sults showed a long distance of genetic linkage between Lr38 and the marker .This band was cloned and sequenced ,the full length 277 bp .This sequence has no homology with the known sequences in GenBank by BLAST ,and maybe a new related sequnce with L r38 .

关 键 词：小麦 抗叶锈基因 LR38 SRAP-SSR(eSSR) 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]