用细胞内注射法观察雏鸡投射向圆核的视顶盖SGC神经元形态  

Morphology of chick SGC neurons projecting to nucleus rotundus observing by intracellular filling method

作　　者：卫雪娇[1] 孙利净 韩秋月[1] 胡满[1] 崔亚利[1] 

机构地区：[1]河北农业大学动物医学院,河北保定071000

出　　处：《河北农业大学学报》2015年第5期68-71,共4页Journal of Hebei Agricultural University

基　　金：国家自然科学基金(30840059)

摘　　要：为了进一步了解鸟类投射向圆核的视顶盖中央灰质层(SGC)神经元的形态特征,向雏鸡圆核内注射快蓝(Fast blue,FB)逆向荧光标记视顶盖SGC神经元,84h后灌流固定,制作300μm厚的中脑振动切片,荧光显微镜下,通过显微操作系统向FB标记的SGC神经元内微电泳法注入荧光染料荧光黄(Lucifer yellow,LY)。结果显示,此法可观察到完整的投射向圆核的SGC神经元胞体和突起。与其它标记法相比,LY细胞内注射法可以标记单一神经元,不会被周围细胞的纤维干扰,是一种研究中枢神经系统神经元间投射关系及其起源细胞的好方法。To study the morphological characteristics of tectal cells in stratum griseum centrale （SGC） which project to nucleus rotundus （Rt） in chick ,SGC neurons were retrogradely la‐beled by injecting a small amount of Fast blue （FB） into Rt .After 84 hours ,the chicks were perfused with fixative and the midbrains were sectioned with vibratome into 300 μm slices . Lucifer yellow （LY） was injected into FB labeled SGC neurons by micro electrophoresis under fluorescence microscopy .The result showed that this method can labele integrative SGC soma and neurites that project to Rt .Contrasting to other labeling methods ,the LY intracellular filling method has the advantages of being able to label a single neuron ,avoiding the interferer by the fibers of other neurons .It is a good method to study the projection between neurons and the morphology of the original cells .

关 键 词：雏鸡 视顶盖 中央灰质层 荧光黄 细胞内注射 

分 类 号：S852.16[农业科学—基础兽医学]