Th17细胞相关因子在Ⅱ型糖尿病小鼠模型中的动态变化  

Dynamic change of Th17-associated factors in mouse model of type-Ⅱdiabetes

作　　者：李强[1] 王玉斌[1] 

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121000

出　　处：《现代预防医学》2015年第19期3564-3567,3571,共5页Modern Preventive Medicine

基　　金：辽宁医学院青年科技启动基金(Y2012Z002);辽宁省大学生创新创业训练计划项(201310160040)

摘　　要：目的检测Ⅱ型糖尿病(T2DM)C57BL/6小鼠外周血及脾脏中白细胞介素-17(IL-17)、β转化生长因子(TGF-β)、白细胞介素-6(IL-6)的变化。方法 C57BL/6小鼠糖尿病造模成功后,于d 3、d 9、d 15、d 21取血及脾脏,用ELISA方法检测小鼠外周血中IL-17、TGF-β、IL-6水平变化及用RT-PCR检测小鼠脾脏IL-17、TGF-β、IL-6 m RNA的表达情况。结果小鼠造模成功后的d 3、d 9、d 15、d 21,外周血IL-17水平在d 3、d 9高于对照组(P<0.05);TGF-β水平在d 3高于对照组(P<0.05),随时间的增长呈下降趋势;IL-6水平呈上升趋势,在d 9、d15高于对照组(P<0.05)。脾脏中IL-17在d 3、d 9高于对照组(P<0.05);TGF-β表达呈下降趋势,在d 3高于对照组(P<0.05);IL-6表达呈上升趋势,在d 9、d 15、d 21高于对照组(P<0.05)。结论Ⅱ型糖尿病C57BL/6小鼠模型中IL-17、TGF-β的表达在造模早期呈升高趋势,IL-6的表达高于对照组,并在d 15达到高峰。该研究结果为Th17亚群在T2DM发生发展进程中发挥的免疫学作用提供了动态的理论数据,并为临床免疫治疗提供理论基础。Objective To investigate the dynamic change of IL-17, TGF- β and IL-6 of the mouse in peripheral blood and spleen, C57BL/6 mice were used to constitute a model of type Ⅱ diabetes. Methods The variation of IL-17, TGF-β and IL-6 concentration in peripheral blood and spleen of successfully established experimental type Ⅱ diabetes models were detected by using the method of ELISA and RT-PCR after 3, 9, 15, 21 days. Results The level of IL-17 in peripheral blood of type Ⅱ diabetes mice was respectively significantly higher than the controlled group in the third and fifth day （P〈0.05）. The level of TGF-β in peripheral blood was significantly higher than the control group in the third day and declined over time （P〈 0.05）. The level of IL-6 in peripheral blood was significantly higher than the control group in the ninth and fifteenth day, and the change was on the rise over time. The expression of IL-17 mRNA in spleen of type Ⅱ diabetes mice was respectively significantly higher than that of the control group in the third and frith day （P〈0.05）. The expression of TGF- β mRNA in spleen was significantly higher than that of the controlled group in the third day and declined over time （P〈0.05）. The expression of IL-6 mRNA was significantly higher than the controlled group in the ninth, fifteenth, twenty-first day and raised over time （P〈0.05）. Conclusion The expressions of IL-17 and TGF-β have an increasing trend in the early of constituting a model of diabetes- Ⅱ mice. The expression of IL-6 is higher than that of the controlled group, and reach a peak in the fifteenth day. The results assist a theoretical dynamic data of immunological role of Th17 subsets in the development of type- Ⅱ diabetes and provide a theoretical basis for clinical immunotherapy.

关 键 词：糖尿病 TH17细胞 IL-17 TGF-Β IL-6 

分 类 号：R181.38[医药卫生—流行病学]