三种不同银纳米粒子SERS基底比较研究  

Comparative Study of Three SERS Active Substrates Based on AgNPs

作　　者：邓悦[1] 吴世法[1] 李睿[1] 丁建华[1] 张毅[1] 

机构地区：[1]大连理工大学物理与光电工程学院,大连116024

出　　处：《光散射学报》2015年第3期231-238,共8页The Journal of Light Scattering

基　　金：国家自然科学基金资助项目(11074029)

摘　　要：表面增强拉曼光谱(SERSp)技术是一种新兴的分析检测技术,由于其对样品分析灵敏度高、检测时间短以及样品所需量小等优点,近年来该技术已在生物医学,化学等领域得到广泛的应用,同时表面增强拉曼散射(SERS)基底的制备已成为该领域的研究热点。本文主要对三种以银纳米粒子(AgNPs)的SERS效应为基质的拉曼活性基底:毛细管-AgNPs,二氧化钛-AgNPs和滤纸-AgNPs进行比较研究。首先分别用三种基底对罗丹明6G(R6G)分子进行拉曼光谱采集及分析,找出三种SERS基底相应的最佳制备条件。最后用这三种最佳条件下制备的SERS基底对同一个健康人血清进行拉曼光谱检测,并对结果进行分析比较。初步结果:三种SERS基底都是可靠的和实用的;二氧化钛-AgNPs基底灵敏度相对较高,但制备过程较复杂;滤纸-AgNPs基底灵敏度其次;毛细管-AgNPs基底及滤纸-AgNPs基底的制备均较为简单。因此,从实用角度考虑,滤纸-AgNPs基底比较适合血清的表面增强拉曼光谱检测与分析。Surface-enhanced Raman spectroscopy technology is an emerging analytical technology. In recent years, with the advantages of high sensitivity, short detection time and small amount of samples, this technology has been widely used in biomedical, chemical and other fields, at the same time, the preparation of SERS substrate has become the research hotspots in this area. In this paper, three SERS active substrates based on AgNPs： capillary-AgNPs, TiO2-AgNPs and filter paper-AgNPs, were used to compare their activities. The optimized preparation condition of above three SERS active substrates are discussed,and their enhanced effects have been compared using R6G as probes. Using three optimized enhanced substrates, the SERS spectra of serum from the same healthy person were detected and analyzed. Preliminary results.these three substrates are reliable and practical,and the sensitivity of TiO2-Ag- NPs is the best,but the prepared process is more complex;the sensitivity of filter paper-Ag- NPs is the next;also, the substrates prepared process of capillary-AgNPs and filter paper-Ag- NPs are easier than that of TiO2-AgNPs. Therefore, from the practical point of view, the filter paper-AgNPs substrates are suitable to the detection and analysis of serum.

关 键 词：表面增强拉曼散射 毛细管 二氧化钛 滤纸 银纳米粒子 血清 

分 类 号：O433.4[机械工程—光学工程]