六味地黄丸中马钱苷大鼠药代动力学研究  

Study on the pharmacokinetic of loganin in Liuwei Dihuang Pills in rats

作　　者：曾庆兰[1] 崔向珍[2] 

机构地区：[1]山东大学附属省立医院妇产科,山东济南250021 [2]山东商业职业技术学院食品药品学院,山东济南250103

出　　处：《药学研究》2015年第10期571-573,598,共4页Journal of Pharmaceutical Research

摘　　要：目的建立液质联用法测定大鼠血浆中马钱苷浓度的方法，研究健康大鼠灌胃六味地黄丸后马钱苷的药代动力学特征。方法6只健康雄性Wistar大鼠，分别按10g·kg^-1。灌胃给予六味地黄丸混悬液，按设定时间点采集血液样苓，采用已建立液质联用法测定血浆中马钱苷浓度。采用DAS2．0药代动力学软件计算药代动力学参数。结果马钱苷主要药代动力学参数分别为t1/2（4．398±1．633）h，Tmax（2．083±1．021）h，Cmax（369．314±53．452）ng·mL^-1，AUC0～24（2275．174±553．825）ng·mL^-1·h，AUC0-∞（2343．478±601．313）ng·mL^-1·h。结论苓研究建立的测定大鼠血浆中马钱苷浓度的液质联用法灵敏度高，重现性好，适于大鼠体内马钱苷血浓度测定及药代动力学研究。大鼠灌胃给予六味地黄丸后，马钱苷药时曲线呈双峰。Objective To establish an HPLC- MS methods for the determination of loganin in rats plasma and to study the pharmacokinetic of loganin after administration of Liuwei Dihuang Pills. Methods 6 Wistar rats were administrated with Liuwei Dihuang Pills suspension( 10 g·kg- 1). The blood samples were collected as scheduled time and analyzed by HPLC- MS. The pharmacokinetic parameters of loganin were calculated by DAS 2. 0 pharmacokinetic software. Results The main parameters of loganin in Liuwei Dihuang Pills were shown as follows: t1 /2( 4. 398 ± 1. 633) h,Tmax( 2. 083 ±1. 021) h,Cmax( 369. 314 ± 53. 452) ng·m L- 1,AUC0 ~ 24( 2 275. 174 ± 553. 825) ng·m L- 1·h,AUC0 ~ ∞( 2 343 ±601. 313) ng·m L- 1·h. Conclusion The HPLC- MS method was sensitive and selective for determination of loganin in plasma. A bimodal phenomenon was observed in the plasma prole after administration of Liuwei Dihuang Pills.

关 键 词：六味地黄丸 马钱苷 药代动力学 液质联用 

分 类 号：R285.5[医药卫生—中药学]