新型鸭呼肠孤病毒TH11株在BHK21细胞系中的增殖特性  

Proliferation characteristics of novel duck reovirus strain TH11 in BHK21 cells

作　　者：丁明洋 毕庄莉 朱英奇[2] 戚伟强[1,2] 朱杰[2] 宋凯杰[2] 陈宗艳[2] 李传峰[2] 吴润[1] 刘光清[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国兽医科学》2015年第9期918-922,共5页Chinese Veterinary Science

基　　金：上海市科委创新项目(13391901600);国家自然科学基金项目(31270194;31101848)

摘　　要：为研究新型鸭呼肠孤病毒（novel duck reovirus,NDRV）在BHK21细胞系中的增殖规律,将NDRV野毒株TH11接种BHK21细胞系,连续传代3次后,观察病毒对细胞的致病变效应（CPE）,并对病毒核酸及其蛋白的表达和病毒增殖特性进行了鉴定。结果显示,NDRV TH11株能够在BHK21细胞中有效增殖,并产生以细胞巨融合为主的典型CPE,病毒蛋白在细胞中也获得了良好表达,并与σC蛋白特异性抗体发生反应。一步生长曲线显示,TH11毒株感染BHK21细胞后第0～12小时为潜伏期,第12小时开始增殖,第12～36小时进入快速增殖期,第48小时病毒效价即达到最高,即TCID50为106.57/0.1mL,随后病毒的增殖随细胞的崩解而降低。上述结果为进一步研究NDRV的致病机理和研制细胞灭活疫苗奠定了基础。To explore the proliferation characteristics of novel duck reovirus（NDRV）in BHK21 cells,BHK21 cells were inoculated with NDRV TH11 strain.After passaged three times,CPE could be observed clearly at hour 12 post-infection.The results of RT-PCR,IFA and Western-blot showed that viral NDRV-σC was expressed well in BHK21 cells,and reacted to the specific antibody against NDRV-σC.One step growth curve revealed that NDRV TH11 strain began to proliferate at hour 12,and reached a rapid proliferation stage during hour 12 to 36post-infection.The virus titer of NDRV TH11 strain reached peak at hour 48.In summary,the above-mentioned results provide some valuable information for further exploring the pathogenesis mechanism of NDRV and vaccine development.

关 键 词：新型鸭呼肠孤病毒 BHK21细胞 增殖特性 

分 类 号：S852.659.4[农业科学—基础兽医学]