枝孢样枝孢霉弱毒株的微波诱变结合亚硝基胍诱变选育  

Breeding of lentogenic Cladosporium cladosporioides mutated by microwave and nitrosoguanidine

作　　者：马晓平[1] 刘艳芳[1] 古玉[2] 曹三杰[1] 邓俊良[1] 黄小波[1] 余树民[1] 左之才[1] 沈留红[1] 伍锐[1] 文翼平[1] 任志华[1] 钟志军[1] 李德生[3] 王承东[3] 凌珊珊[3] 杨天意[1] 向奇 叶娇[1] 杨秋霞[1] 童渝斐 侯蓉 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川成都611130 [2]四川农业大学生命科学院,四川成都611130 [3]中国保护大熊猫研究中心,四川雅安625000 [4]成都大熊猫繁育研究基地,四川成都610081

出　　处：《中国兽医科学》2015年第9期923-929,共7页Chinese Veterinary Science

基　　金：国家科技支撑计划项目(2012BAC01B06);四川省科技厅基础研究计划项目(2013JY0175);四川省教育厅青年基金项目(12ZB093);大熊猫国际资金项目(AD1415)

摘　　要：为建立枝孢样枝孢霉弱毒株突变体的诱变方法,研究枝孢样枝孢霉及其突变体生物特性和致病力,用不同浓度的亚硝基胍和微波诱变野生型枝孢样枝孢霉,经小鼠致病性试验筛选,得到1株菌落颜色明显变浅,表型改变,产孢期短,产孢量明显减少,对小鼠致病力减弱的毒株Zt。确定了枝孢样枝孢霉突变体的诱变条件,得到了预期突变样本。The mutational method of Cladosporium cladosporioides with hypotoxicity was studied.The mutant of C.cladosporioides with hypotoxicity was obtained by mutagen nitrosoguanidine with different concentrations and microwave selection from wild-type C.cladosporioides.Finally,a weak toxicity strain（Zt）was selected with light color,phenotypic change,thin hyphae,spore yields less and low pathogenicity to mouse relative to wild-type C.cladosporioides.The mutagenesis conditions of C.cladosporioides with hypotoxicity and the expected mutant of C.cladosporioides for comparing the differences between genes was obtained.

关 键 词：枝孢样枝孢霉 诱变 突变体 形态 致病性 

分 类 号：S852.42[农业科学—基础兽医学]