糖基化终末产物对小鼠骨髓间充质干细胞增殖及相关基因P27、cyclinD1的影响  

Effects of Advanced Glycation End Products on the Proliferation of Mouse Bone Marrow Mesenchymal Stem Cells and Expression of P27 and Cyclin D1

作　　者：孙传锋[1] 邓超[2] 印奇志[1] 柳海[2] 周嵩琳[2] 徐清[2] 

机构地区：[1]六安市人民医院口腔科,安徽六安237005 [2]皖南医学院

出　　处：《口腔医学研究》2015年第9期880-882,886,共4页Journal of Oral Science Research

基　　金：皖南医学院重点科研项目培育基金(编号:WK2013Z10)

摘　　要：目的:探讨糖基化终末产物(AGEs)对小鼠骨髓间充质干细胞(BMSCs)增殖能力以及相关基因P27、cyclinD1的影响。方法:全骨髓法培养小鼠骨髓间充质干细胞;成骨、成脂诱导骨髓间充质细胞,对其进行干细胞鉴定;将培养出的骨髓间充质干细胞与不同浓度的AGEs共培养,MTT检测不同浓度下骨髓间充质干细胞增殖的改变;实时定量聚合酶链反应(Real time PCR)检测AGEs刺激后P27、cyclin D1表达的改变。结果:小鼠骨髓间充质干细胞成骨诱导21d后茜素红染色出现钙化结节;成脂诱导21d后油红O染色出现脂滴;MTT显示不同浓度的AGEs(10、50、100、200mg/L)均能抑制BMSCs的增殖差异有统计学意义(P<0.05),且随着浓度的增加抑制越明显;Real time PCR结果显示:AGEs(50mg/L)刺激3d后P27mRNA的表达水平较对照组升高,cyclinD1mRNA的表达水平较对照组降低,差异有统计学意义(P<0.05)。结论:AGEs能抑制小鼠骨髓间充质干细胞的增殖并能改变P27、cyclinD1mRNA的表达。Objective： To investigate the effects of advanced glycation end products （AGEs） on the proliferation of mouse bone marrow mesenehymal stem ceils （BMSCs）. Methods： BMSCs were adopted by whole bone marrow adherence method. Osteogenic differentiation capacity of BMSCs was evaluated by Alizarin red staining. Adipogenic differentiation capacity of BMSCs was evaluated by Oil red staining. After induced with different concentrations of AGEs, the proliferation of BMSCs was assayed by MTT. In addition, real--time quantitative reverse transcription polymerase chain reaction （real-time PCR） was performed to detect the gene expression levels. Results： After 21 -day induction, Alizarin red staining showed the formation of mineralization nodules and oil red staining showed the formation of lipid droplets, All the different concentrations of AGEs （10μg/mL, 50μg/mL, 100μg/mL, 200μg/ mL） obviously inhibited the proliferation of BMSCs. Meanwhile, the expressions of P27 and eyclinD1 in the experimental group was significantly different from that in the control group （P〈0.05）. Conclusion： AGEs could decrease the proliferation capacity of BMSCs and regulate the gene expression levels of P27 and cyclin D1.

关 键 词：糖基化终末产物 小鼠骨髓间充质干细胞 增殖 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]