HPLC法测定补肾化瘀浸膏中毛蕊花糖苷的含量  

Determination of acteoside in Bushenhuayu extracts by HPLC

作　　者：王娟[1,2] 汪选斌[1,2] 李洪亮[1,2] 欧阳露[1,2] 

机构地区：[1]湖北医药学院附属人民医院中药药理实验室,湖北十堰442000 [2]湖北医药学院药学院,湖北十堰442000

出　　处：《实用药物与临床》2015年第9期1081-1083,共3页Practical Pharmacy and Clinical Remedies

基　　金：湖北省教育厅项目(B2015487);湖北省2011协同创新中心基金(4);湖北省自然科学基金面上项目(2014CFB642)

摘　　要：目的 建立补肾化瘀浸膏中毛蕊花糖苷的HPLC含量测定方法. 方法 采用ODS Phenomenex Gemini C18柱（250 mm × 4. 6 mm,5 μm）,以乙腈-0.1%醋酸溶液（16：84）为流动相,检测波长：334 nm,流速：1. 0 mL/min,柱温：30 ℃. 结果 毛蕊花糖苷进样量在0.015 ~0.300 μg 之间线性关系良好. 回归方程 Y =16 763 X-7 948. 9,R=0.999 6;平均加样回收率为98. 24%,RSD为1. 34%（n=6）. 结论 本方法操作简便,测定结果准确,重复性好,可作为补肾化瘀浸膏的质量控制方法.Objective To establish a method for determination of acteoside in Bushenhuayu extracts by HPLC. Methods HPLC was conducted at a column temperature of 30 ℃ with a column of ODS Phenomenex Gemini C18（250 mm × 4. 6 mm,5 μm）,the mobile phase consisted of acetonitrile-0.1% acetic acid （16：84 V/V）,wave length was set at 334 nm and the flow rate was 1. 0 mL/min. Results There was a good linear ranging from 0.015~0.300 μg of acteoside. The curve formulation was Y=16 763 X-7 948. 9,R=0.999 6,with an average recovery of 98. 24% （RSD=1. 34%,n=6）. Conclusion The method might be one of the good quality criterion for Bushenhuayu extract because of its simple,accurate and recovery property.

关 键 词：高效液相色谱法 补肾化瘀浸膏 毛蕊花糖苷 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]