褪黑素对过度训练大鼠胃肠动力及血浆中应激激素水平的影响  

The experimental study on melatonin gastro intestinal motility and plasma levels of stress hormones in overtraining rat

作　　者：景会锋[1] 王小梅[1] 

机构地区：[1]延安大学体育学院,陕西延安716000

出　　处：《中国应用生理学杂志》2015年第5期411-413,共3页Chinese Journal of Applied Physiology

摘　　要：目的:探讨褪黑素对过度训练大鼠胃肠动力及血浆中应激激素水平的影响。方法:成年SD大鼠30只,随机分为对照组、过度训练组、褪黑素干预组(n=10)。在每次训练前30 min对照组和过度训练组大鼠灌服生理盐水(15 ml/kg)、褪黑素干预组大鼠灌服褪黑素(15 ml/kg),每天1次,每周5次。过度训练组和褪黑素干预组大鼠进行每周5次,共6周的过度训练。6周后,观察各组大鼠胃内残留率、小肠推进比、血浆中胃动素(MTL)、降钙素基因相关肽(CGRP)、皮质醇(CORT)和儿茶酚胺(CA)水平。结果:与对照组相比,过度训练组大鼠胃内残留率、小肠推进比、血浆中MTL、CORT和CA水平明显升高(P<0.01),血浆中CGRP浓度明显降低(P<0.01),经过褪黑素干预后,大鼠胃内残留率、小肠推进比、血浆中MTL、CORT和CA水平明显降低(P<0.01),血浆中CGRP浓度明显升高(P<0.01)。结论:褪黑素对过度训练引起的胃肠道功能紊乱具有保护作用,MTL、CGRP、CORT和CA在其发病中均起重要作用。Objective： To study the effect of melatonin on the gastrointestinal motility and plasma levels of the stress hormone in overtrain- ing rats. Method： Thirty adult SD rats were randomly divided into three groups（ n -- 10） ： control group, over-training group, melatonin inter- vention group. 30 min before each training, rats in the control and over-training groups were fed with normal saline （15 mg/kg） once a day and 5 times per week, while rats in the melatonin intervention group were administrated with melatonin, perfusion in the intervention group （15 mg/kg）. Excessive training group and melatonin intervention group rats were subjected to excessive training at 5 times a week for 6 weeks. Af- ter 6 weeks, the gastric emptying rate, small intestinal propulsion ratio and levels of plasma motilin （MTL） and calcitonin gene-related peptide （CGRP）, cortisol （CORT） and catecholamines （CA） were observed in all groups. Results： Compared with the control group, the gastric emptying rate, small intestinal propulsion ratio and levels of plasma MTL, CORT and CA were increased significantly （ P 〈 0.01 ） while the content of CGRP was reduced （ P 〈 0.01） in over-training group. After treated with melatonin, this trend was reversed, that was, the gastric emptying rate, small intestinal propulsion ratio and levels of plasma MTL, CORT and CA were surpressed significantly （ P 〈 0.01 ） while the content of CGRP was improved obviously （P 〈 0.01） in over-training group. Conclusion： Melatonin plays an important role in protecting gas- trointestinal tract from dysfunction, in which MTL, CGRP, CORT and CA are all involved.

关 键 词：褪黑素 过度训练 胃肠动力 应激激素 大鼠 

分 类 号：G804.7[文化科学—运动人体科学]