血红素氧合酶-1对大鼠肝脏缺血再灌注NF-κBp65和细胞凋亡的影响  

Effects of heme oxygenase-1 on NF-κB p65 and apoptosis in rats during the liver ischemia / reperfusiondamage

作　　者：吕飒美[1] 吴友伟[1] 周健[1] 史丽萍[1] 

机构地区：[1]陕西省人民医院消化内2科,西安710068

出　　处：《中国临床保健杂志》2015年第5期506-508,I0002,共4页Chinese Journal of Clinical Healthcare

摘　　要：目的探讨血红素氧合酶-1(HO-1)对大鼠肝脏缺血再灌注NF-κB P65、细胞凋亡的影响。方法SD大鼠采用随机数字表法分成5组:正常组(N),假手术组(S),手术组(I/R建立肝脏缺血再灌注损伤模型),手术给药Ⅰ组(I/R+hemin),手术给药Ⅱ组(I/R+Zn PP),手术后6 h、12 h下腔静脉取血分离血清检测血清谷草转氨酶(AST)、谷丙转氨酶(ALT)。Western blot方法测定肝脏NF-κB p65的表达,免疫组化方法测定肝脏Fas蛋白的表达。结果 1hemin组ALT、AST较I/R组及Zn PP组明显降低(P<0.01);2肝组织NF-κB p65的表达:hemin组NF-κB p65表达比同期I/R组减少(P<0.01);Zn PP组NF-κB p65表达比同期I/R组增多(P<0.01);3肝组织Fas:hemin组Fas表达减少(P<0.05),Zn PP组Fas表达明显增多(P<0.01)。结论 HO-1对肝脏缺血/再灌注损伤的保护作用与NF-κBP65密切相关,可能通过抑制NF-κB p65的活性来减少细胞凋亡。Objective To explore the effects of Heme Oxygenase-1 on NF-κB P65 and apoptosis in rats dur- ing the liver ischemia/eperfusion damage. Methods SD rats were randomly divided into five groups ： normal controlgroup, sham group, I/R group, I/R ＋ hemin group, and I/R ＋ ZnPP group. Blood samples were collected from the postcava at 6h andl2h after the operation, and the serum was isolated to measure alanine aminotransferase （ALT） and aspartate aminotransferase （AST）. The expressions of NF-κBp65 and Fas protein in livers of rats were examined by western blot and immunohistochemistry. Results ①The levels of ALT and AST were significantly lower in hemin group than those in I/R group and ZnPP group（ P 〈 0.01 ）. ②The expression of NF-κB p65 was lower in hemin group than those in I/R group（ P 〈 0. 01 ） ; the expression of NF-κB p65 was higher in ZnPP group than those in I/R group （ P 〈 0.05 ）. ③The expression of Fas protein was markedly increased in ZnPP group than those in hemin group （ P 〈 0. 01 ）. Conclusion Protective effects of HO-1 against hepatic ischemia/reperfusion damage were closely related to the reduced NF-κB P65 expression,which maybe decreased the apoptosis via inhibit activity of NF-κBp65.

关 键 词：肝疾病 再灌注损伤 p65亚基NF—κB 氧合酶类 大鼠 sprague—dawley 

分 类 号：R657.3[医药卫生—外科学]