基于Pd/COF-LZU1非标记型C-反应蛋白免疫传感器的研制  

Preparation of Label-free C-Reactive Protein Immunosensor Based on the Palladium-coordinated Covalent Organic Frameworks(Pd/COF-LZU1) Material

作　　者：刘婷知 夏介仁 李瑶[1] 陈文凯[1] 张帅[1] 刘仪[1] 郑丽[1] 杨云慧[1] 

机构地区：[1]云南师范大学化学化工学院,昆明650500

出　　处：《高等学校化学学报》2015年第10期1880-1887,共8页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21465026;21165023)资助~~

摘　　要：采用溶剂热法,通过有机单体合成了一种亚胺键连接的共价有机框架材料（COF-LZU1）;在常温常压条件下,通过后合成的方法将贵金属钯（Ⅱ）引入到COF材料中,合成了复合材料Pd/COF-LZU1,该材料具有优良的催化性能.利用Pd/COF-LZU1多孔复合材料将C-反应蛋白（CRP）抗体（anti-CRP）固定在玻碳电极表面,构建了一种非标记型CRP免疫传感器.当抗体与抗原发生免疫反应时,形成的免疫复合物会阻碍电化学探针[Fe（CN）6]^4-/3-的电子传递,降低其响应电流,从而实现CRP的快速检测.采用交流阻抗和差示脉冲伏安法（DPV）考察了免疫传感器的电化学特性,同时考察了测试底液的p H值、抗原培育时间和抗体固定浓度等实验条件对传感器性能的影响.在最优的实验条件下,采用DPV法对CRP进行检测的线性范围为5-180 ng/m L,检出限为1.66 ng/m L,线性相关系数为0.992.A new imine-linked covalent organic framework（ COF） material was synthesized using organic monomer via solvothermal methods. Pd（ Ⅱ）-coordinated COF,Pd / COF-LZU1,was accordingly synthesized through simple post-treatment at ambient conditions,which showed excellent catalytic activity. Then,a labelfree C-reactive protein（ CRP） immunosensor was constructed using the Pd / COF-LZUI porous composite material to immobilize anti-CRP on the surface of GCE. When anti-CRP binds with CRP antigen,the formed immuno complex obstruct the electric signal of Fe（ CN）6^4-/3-,which can be used to realize label-free detection of CRP. Electrochemical impedence spectroscopy（ EIS） and differential pulse voltammetry（ DPV） were employed to characterize the immunosensor. The experimental conditions such as scanning rate,p H value of testing solution,the concentration of anti-CRP and incubation time were also optimized. Under the optimal experimental conditions,the linear range was 5—180 ng / m L,the detection limit was 1. 66 ng / m L,with the linear correlation coefficient of 0. 992. This immunosensor provides a novel and simple way to detect CRP,and develops the application of the COFs in the electrochemical immunosensor.

关 键 词：钯负载共价有机框架材料 C-反应蛋白 免疫传感器 非标记型 

分 类 号：O657.1[理学—分析化学]