基于WST-8细胞活性实验的表面增强拉曼光谱研究  

WST-8 Assay for Living Cell Viability Detection by Surface-enhanced Raman Spectroscopy

作　　者：杨津[1,2] 刘卓[3] 王晓慧[2] 隋会敏 毛竹[1] 赵冰[1] 

机构地区：[1]吉林大学超分子结构与材料国家重点实验室,长春130012 [2]吉林大学基础医学院 [3]吉林大学中日联谊医院,长春130021

出　　处：《高等学校化学学报》2015年第10期1900-1905,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21273091;21221063;21327803;2141114023);吉林省科技厅青年基金(批准号:20130522181JH)资助~~

摘　　要：采用常规的用于测定活细胞个数的试剂2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐(WST-8四唑盐)建立了一种快速测定细胞活性的表面增强拉曼散射光谱(SERS)新方法.考察了p H值及还原剂烟酰胺腺嘌噙二核苷酸(NADH)用量对WST-8甲瓒信号的影响.结果表明,与传统的WST-8比色法相比,SERS法具有更高的检测灵敏度和选择性,对WST-8甲瓒的检测限可达到10μmol/L.Surface-enhanced Raman scattering（ SERS） technique was used to develop and optimize a novel and quantitative 2-（ 2-methoxy-4-nitrophenyl）-3-（ 4-nitrophenyl）-5-（ 2,4-disulfophenyl）-2H-tetrazolium monosodium salt（ WST-8） assay for living cell viability. The influences of p H and dosage of reductant nicotinamide adenine dinucleotide（ NADH） on WST-8 formazan signal were examined. SERS signals of formazan with high resolution and great diversity between WST-8 and formazan were observed in neutral conditions.Meanwhile,the signals of formazan（ 560,1351,1598,1616 cm-1） could be found clearly when the concentration of NADH was four times that of WST-8. Compared with the traditional method,this assay appears higher sensitivity and selectivity for WST-8 formazan. Moreover,the detection limit of formazan is as low as 10μmol /L.

关 键 词：2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2 4-二磺基苯)-2H-四唑单钠盐(WST-8) 表面增强拉曼光谱 细胞活性检测 

分 类 号：O657.37[理学—分析化学]