大生产中丹参提取物制备工艺优选  

Optimization Preparation Process of Salviae Miltiorrhizae Radix et Rhizoma Extract in mass production

作　　者：徐玉玲[1] 梁悦[1] 赵群[1] 张文文[1] 聂忠莉[1] 刘涛[1] 

机构地区：[1]成都大学生物工程学院,成都610106

出　　处：《中国实验方剂学杂志》2015年第19期24-27,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省中医药管理局中医药科学技术研究专项(2014F004);四川省科技厅丹参整合式全产业链综合开发研究项目(2014SZ0136)

摘　　要：目的：对2010年版《中国药典》一部中丹参的2种提取物的制备工艺参数进行讨论,以便其更适应大生产的需要。方法：采用单因素试验法,以隐丹参酮、丹参酮ⅡA的含量为指标,探讨丹参酮提取物原料的前处理方法;以固含物总量、丹酚酸B含量为指标考察丹参水提的最佳温度;以固含物总量、丹酚酸B含量、指纹图谱为指标确定丹参水提的原材料;运用指纹图谱技术确定丹参水提液的最佳浓缩温度。结果：丹参酮提取物原料的前处理方式可由粗粉变更为切成1~2 cm的小段;丹参总酚酸提取物的原材料可采用丹参酮提取物的药渣,提取温度可由80℃修订为100℃,浓缩温度可由60℃修订为80℃。结论：优选的工艺条件稳定可行,可为丹参提取物的大生产参数修订提供实验依据。Objective： To discuss preparation process parameters of Salviae Miltiorrhizae Radix et Rhizoma extract in mass production. Method： Single factor tests were adopted, pretreatment of raw materials of tanshinone extract was investigated by taking contents of cryptotanshinone and tanshinone HA as indicators. Water extraction temperature of Salviae Miltiorrhizae Radix et Rhizoma was optimized by taking solid-containing content and the content of salvianolic acid B as indexes. With solid-containing content, the content of salvianolic acid B and fingerprints as indicators, raw materials of water extraction for Salviae Miltiorrhizae Radix et Rhizoma were determined. Fingerprints was employed to determine optimum concentration temperature of water extract of Salviae Mihiorrhizae Radix et Rhizoma. Result： Pretreatment of raw materials of tanshinone extract was cutting into small pieces of 1-2 cm. Raw materials of total phenolic acid extract in Salviae Miltiorrhizae Radix et Rhizoma can use the residues of tanshinone extract, extraction temperature was revised to 100 ℃, the concentrated temperature was revised to 80 ℃. Conclusion： These optimized process conditions are stable and feasible, which can provide experimental basis for large-scale production of Salviae Mihiorrhizae Radix et Rhizoma extract.

关 键 词：丹参 丹参酮提取物 丹酚酸B 隐丹参酮 指纹图谱 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]