洋川芎内酯I在大鼠尿液和胆汁中的排泄  

Urine and Bile Excretion of Senkyunolide I in Rats

作　　者：马聪[1] 熊耀坤[2] 马诗瑜[3] 富臻 李俊[1] 梁爽[1] 冯怡[1] 

机构地区：[1]上海中医药大学中药现代制剂技术教育部工程中心,上海201203 [2]江西中医药大学,南昌330004 [3]上海中医药大学中药学院,上海201203

出　　处：《中国实验方剂学杂志》2015年第19期75-79,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家教育部科学技术研究重点项目(211060);国家教育部高等学校博士学科点专项科研基金项目(20123107120009)

摘　　要：目的:研究洋川芎内酯I在灌胃和静脉注射后在大鼠体尿液和胆汁中的排泄特征,为该成分的临床前研究和评价提供参考。方法:大鼠按剂量72 mg·kg-1灌胃和尾静脉给与洋川芎内酯I溶液,收集不同时间段尿液和胆汁,采用高效液相色谱-紫外检测器法(HPLC-VWD)测定大鼠尿液和胆汁中洋川芎内酯I的含量,检测波长均为278 nm,流动相均为乙腈-0.1%甲酸水溶液梯度洗脱。结果:灌胃和尾静脉给药48 h后累积经尿液排泄的原形药物量分别为(179.8±33.68),(264.8±87.28)μg,累积排泄率分别为(0.77±0.15)%和(1.35±0.26)%;给药36 h后累积经胆汁排泄的原形药物量依次为(359.4±75.66),(426.3±140.90)μg,累积排泄率分别为(1.22±0.49)%和(1.72±0.59)%。结论:洋川芎内酯I在大鼠尿液和胆汁内以原形药物的形式排出量较少,且排泄迅速。Objective： To study excretion characteristics of senkyunolide I in urine and bile by intravenous injection and intragastric administration, respectively. Method： Rats were intravenous injected and intragastric administrated with senkyunolide I at a dose of 72 mg .kg-1, respectively. The urine and bile were collected at different time points. The concentrations of senkyunolide I in urine and bile were measured by HPLC- VWD, detection wavelength was set at 278 nm, mobile phase consisted of acetonitril-water （containing 0. 1% formic acid） for gradient elution. Result： After intragastric administration and intravenous injection, cumulative excretion of senkyunolide I in 48 h were （179. 8±33.68）, （264.8 ±87.28） μg in urine, cumulative excretion percentages were calculated to be （0.77 ± 0. 15）% and （1.35 ±0.26）% , respectively; cumulative excretion of senkyunolide I in 36 h were （359.4 ±75.66）, （426.3 ± 140.90） μg in bile, cumulative excretion percentages were calculated to be （1.22 ±0.49） % and （ 1.72 ± 0.59） % , respectively. Conclusion： Prototype is not main elimination form of senkyunolide I in rats＇ urine and bile, its speed of excretion is fast.

关 键 词：洋川芎内酯I 累积排泄率 尿液 胆汁 川芎 

分 类 号：R969.1[医药卫生—药理学] R945[医药卫生—药学]