中风胶囊对动脉粥样硬化大鼠血脂及相关细胞因子的影响  

Effect of Zhongfeng Capsule on Blood Fat and Cytokines in Rats with Atherosclerosis

作　　者：马杰[1] 黄霞[2] 刘惠霞[2] 孙为[2] 

机构地区：[1]郑州师范学院生物工程研究所,郑州450044 [2]河南省中医药研究院中药研究所,郑州450004

出　　处：《中国实验方剂学杂志》2015年第19期101-105,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省中医药研究院中医心病专项科研基金项目(1304489)

摘　　要：目的:观察中风胶囊(ZFJN)对动脉粥样硬化(AS)模型大鼠血脂及相关细胞因子的影响。方法:SD大鼠随机分为正常组,模型组,中风胶囊低、高剂量组(1.5,3.0 g·kg-1),阿托伐他汀钙片(阿乐,5 mg·kg-1)组。正常组动物摄食正常颗粒饲料,其余组在摄食高脂饲料的基础上采用ip维生素D3加激发免疫反应的方法复制AS模型,各组动物连续ig相应药物90 d,检测血清胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C),白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α),白细胞介素-18(IL-18),脂联素(APN),可溶性CD40L(s CD40L)并计算动脉硬化指数(AI);取主动脉进行HE染色,观察组织病理学变化。结果:与正常组比较,模型组大鼠血清TC,LDL-C,HDL-C,TNF-α,IL-18,s CD40L及AI明显升高(P<0.05,P<0.01),而APN水平明显降低(P<0.01);与模型组比较,ZFJN高剂量组均明显降低大鼠血清LDL-C,TNF-α,IL-18以及AI水平(P<0.05,P<0.01),明显升高血清APN水平(P<0.01);HE染色显示模型组主动脉可见内皮损伤和粥样斑块,给药各组均可抑制其主动脉损伤。结论:ZFJN对大鼠AS的发生发展具有一定干预作用,其作用环节与降低血清LDL-C含量,提高APN水平,减少炎性细胞因子TNF-α,IL-18的分泌或释放有关。Objective： To investigate the effect of the Zhongfeng capsule on blood fat and cytokines in rats with atherosclerosis. Method： SD rats were randomly divided into 5 groups： the normal group, the model group, the high-, low-dose Zhongfeng capsule groups and the positive group （atorvastatin calcium tablets, 5 mg .kg-1 ）. The rats in the normal group received saline and basic food. The rest rats were injected with VD3 and ovalbumin, while fed with a high-fat diet. All rats received the corresponding drugs for 90 days. The levels of total cholesterol （TC）, triglyceride （TG）, low density lipoprotein cholesterol （LDL-C） and high density lipoprotein cholesterol （HDL-C）, interleukin-6 （IL-6）, interleukin-18 （IL-18）, tumor necrosis factor-α （TNF-α）, adiponectin （APN） and soluble CD40L （sCD4OL） in the blood serum were analyzed and the arteriosclerosis index （AI） were calculated. The pathological observation of aorta was carried out. Result： Compared with normal group, the levels of TC, LDL-C, HDL-C, TNF-α, IL-18, sCDgOL and AI were significantly increased, while the APN decreased in the model group （P 〈0.05, P 〈0.01）. Compared with the model group, the AI and LDL-C, TNF-α, IL-18 levels decreased, while the serum APN level increased in the high-dose Zhongfeng capsule group （P 〈 0.05, P 〈 0. 01）. HE stain showed aortic endothelial injury and atherosclerotic plaque formation in the model group, while the aortic lesions could be restrained in different degree in the treated groups. Conclusion： Zhongfeng capsule could inhibit the atherogenesis formation and development in atherosclerosis. The mechanisms may be involved inreducing the level of serum LDL-C, improving the level of APN and decreasing the secretion or release of the inflammatory factors.

关 键 词：中风胶囊 动脉粥样硬化 细胞因子 

分 类 号：R285.5[医药卫生—中药学]