当归双参蛤蟆油片对去势大鼠内分泌的调节作用  

Effect of Danggui Shuangshen Hamayou Tablets in Regulating Incretion in Ovariectomized Rats

作　　者：刘凡 李俊葵[2] 雷晓林 

机构地区：[1]东方药林药业有限公司,广州510515 [2]南方医科大学,广州510515

出　　处：《中国实验方剂学杂志》2015年第19期125-128,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省教育部产学研结合项目(2007B090400026)

摘　　要：目的:观察当归双参蛤蟆油片对去势雌性大鼠内分泌的调节作用。方法:将SD雌性50只大鼠进行双侧卵巢切除术后,随机分为5组:模型组、利维爱组、当归双参蛤蟆油片高、中、低剂量组(0.6,0.3,0.15 g·kg-1),每组10只,另取10只作为假手术组,各给药组灌服相应药液,模型组和假手术组灌服生理盐水,连续30 d。处死动物,取大鼠脑、子宫、肾上腺,计算其脏器指数,并用试剂盒检测雌二醇(E2),促黄体生成素(LH),促卵泡激素(FSH)含量。采用化学荧光检验法测定去甲肾上腺素(NE),多巴胺(DA),5-羟色胺(5-HT)及5-羟吲哚乙酸(5-HIAA)含量。结果:与假手术组比较,模型组大鼠的体重、血清E2,子宫指数,肾上腺素指数水平及NE,DA含量均明显降低(P<0.05,P<0.01),LH,FSH,5-HT,5-HIAA含量明显升高(P<0.05,P<0.01);与模型组比较,当归双参蛤蟆油片能明显增大模型组大鼠体重,当归双参蛤蟆油片各剂量均能明显提高模型大鼠血清E2水平(P<0.05),且对LH,FSH无影响,各组用药对去势后降低的子宫指数均有明显升高作用(P<0.05);当归双参蛤蟆油片高剂量能升高去势后降低的肾上腺指数(P<0.05)。各组用药对去势大鼠升高的5-HT,5-HIAA的含量均有明显降低作用(P<0.05),当归双参蛤蟆油片高、中剂量均能明显升高大鼠去势后降低的NE,DA水平(P<0.05)。结论:当归双参蛤蟆油片具有调节生殖内分泌激素和下丘脑神经递质的作用。Objective： To observe the effect of Danggui Shuangshen Hamayou tablets （DSHT） in regulating the endocrine system of ovariectomized rats. Method： Totally 50 ovariectomized female rats were randomly divided into five groups： model group, Livial group and DSHT high, middle and low dose groups （0.6, 0.3, 0. 15 g .kg-1 ） , with 10 in each group. Another 10 were included into the sham group. Each treatment group was fed with the corresponding liquid. Model group and sham group were orally given normal saline for 30 days. Animals were killed, rat brain, uterus and adrenal were collected to calculate the viscera index, and detect estradiol （E2）, luteinizing hormone （LH）, follicle-stimulating hormone （FSH） with content kit. Norepinephrine （ NE）, dopamine （ DA）, 5-hydroxytryptamine （5-HT） and 5-hydroxyindole acetic acid （5-HIAA） content were tested by chemical fluorescence. Result： Compared with the sham group, the model group showed significantly lower weight, serum E2 , uterus index, adrenaline index, NE, DA content （P 〈 0.05, P 〈 0.01 ） and higher LH, FSH, 5-HT, 5-HIAA content （P 〈 0.05, P 〈 0.01 ）. DSHT could significantly increase the weight of rats in the model group, each DSHT group could significantly increased serum E2 levels in rats （P 〈 0. 05） , with no effect on LH, FSH. Each treatment group showed significantly increase in the lowered uterus index after castration （P 〈 0.05） , and high dose of DSHT could increase the lowered adrenal index after castration （P 〈 0.05）. Each groupshowed significantly reduction in the increased high-dose and the middle-dose of DSHT could castration （P 〈 0.05 ）. Conclusion： DSHT neurotransmitters. 5-HT, 5-HIAA content in significantly elevated the can regulate reproductive ovariectomized rats （ P 〈 0.05 ）. The high-dose and the middle-dose of DSHT could significantly elevated the reduced NE, DA levels in rats aftercastration （P 〈 0.05 ）. Conclusion： DSHT can regulate reproductive end

关 键 词：当归双参蛤蟆油片 双侧卵巢切除术 生殖内分泌激素 脏器指数 下丘脑神经递质 

分 类 号：R285.5[医药卫生—中药学]